| Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase. | |
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MedLine Citation:
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PMID: 17512460 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Repair of the oxidized purine 8-oxo-7,8-dihydroguanine (8-oxoGua) is inefficient in cells belonging to the B complementation group of Cockayne syndrome (CS-B), a developmental and neurological disorder characterized by defective transcription-coupled repair. We show here that cells belonging to the A complementation group (CS-A) are also defective in repair of 8-oxoGua and we demonstrate that expression of the Escherichia coli formamidopyrimidine DNA glycosylase (FPG) completely corrects the repair deficiency in both CS-A and CS-B cells. Phenotypically, CS-A cells are normally sensitive to toxicity and micronuclei induced by the oxidizing agent potassium bromate. CS-B cells display sensitivity to elevated concentrations of potassium bromate but this is not compensated by FPG expression, suggesting toxicity of lesions that are not FPG substrates. The data indicate that 8-oxoGua is not a major toxic and clastogenic lesion in CS cells. |
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Authors:
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Monica Ropolo; Paolo Degan; Mara Foresta; Mariarosaria D'Errico; Denise Lasigliè; Eugenia Dogliotti; Gianluigi Casartelli; Simonetta Zupo; Alessandro Poggi; Guido Frosina |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-12 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 42 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-05-21 Completed Date: 2007-07-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 1807-17 Citation Subset: IM |
Affiliation:
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Experimental Oncology B Laboratory, Department of Translational Oncology, Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n. 10, 16132 Genova, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Bromates / pharmacology Carcinogens / pharmacology Cell Survival / drug effects Cells, Cultured / drug effects Cockayne Syndrome / genetics* Colony-Forming Units Assay DNA Damage* DNA Repair* DNA-Formamidopyrimidine Glycosylase / genetics, metabolism* Escherichia coli / enzymology* Female Fibroblasts / drug effects Genetic Complementation Test* Genetic Vectors Humans Kidney / metabolism, pathology Male Micronucleus Tests Transcription, Genetic Urinary Bladder Neoplasms / metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Bromates; 0/Carcinogens; 7758-01-2/potassium bromate; EC 3.2.2.23/DNA-Formamidopyrimidine Glycosylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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