Document Detail

Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase.
MedLine Citation:
PMID:  17512460     Owner:  NLM     Status:  MEDLINE    
Repair of the oxidized purine 8-oxo-7,8-dihydroguanine (8-oxoGua) is inefficient in cells belonging to the B complementation group of Cockayne syndrome (CS-B), a developmental and neurological disorder characterized by defective transcription-coupled repair. We show here that cells belonging to the A complementation group (CS-A) are also defective in repair of 8-oxoGua and we demonstrate that expression of the Escherichia coli formamidopyrimidine DNA glycosylase (FPG) completely corrects the repair deficiency in both CS-A and CS-B cells. Phenotypically, CS-A cells are normally sensitive to toxicity and micronuclei induced by the oxidizing agent potassium bromate. CS-B cells display sensitivity to elevated concentrations of potassium bromate but this is not compensated by FPG expression, suggesting toxicity of lesions that are not FPG substrates. The data indicate that 8-oxoGua is not a major toxic and clastogenic lesion in CS cells.
Monica Ropolo; Paolo Degan; Mara Foresta; Mariarosaria D'Errico; Denise Lasigliè; Eugenia Dogliotti; Gianluigi Casartelli; Simonetta Zupo; Alessandro Poggi; Guido Frosina
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-12
Journal Detail:
Title:  Free radical biology & medicine     Volume:  42     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-21     Completed Date:  2007-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1807-17     Citation Subset:  IM    
Experimental Oncology B Laboratory, Department of Translational Oncology, Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n. 10, 16132 Genova, Italy.
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MeSH Terms
Aged, 80 and over
Bromates / pharmacology
Carcinogens / pharmacology
Cell Survival / drug effects
Cells, Cultured / drug effects
Cockayne Syndrome / genetics*
Colony-Forming Units Assay
DNA Damage*
DNA Repair*
DNA-Formamidopyrimidine Glycosylase / genetics,  metabolism*
Escherichia coli / enzymology*
Fibroblasts / drug effects
Genetic Complementation Test*
Genetic Vectors
Kidney / metabolism,  pathology
Micronucleus Tests
Transcription, Genetic
Urinary Bladder Neoplasms / metabolism,  pathology
Reg. No./Substance:
0/Bromates; 0/Carcinogens; 7758-01-2/potassium bromate; EC Glycosylase

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