Document Detail


Complementation of a fibre mutant adenovirus by packaging cell lines stably expressing the adenovirus type 5 fibre protein.
MedLine Citation:
PMID:  9634089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adenovirus-based gene therapy vectors now in use cannot be targeted to specific cell types in vivo and are immunogenic, properties which limit their clinical utility. Improved vectors lacking the genes for viral structural proteins may overcome these limitations. We have developed cell lines which stably express the adenovirus type 5 (Ad5) fibre protein in its native trimeric form. These cells can complement an Ad5 mutant with a defect in the fibre gene, and are capable of incorporating the Ad5 fibre into particles of a different Ad serotype. As the fibre protein is responsible for the initial binding of virus to cells, packaging cell lines expressing different or modified fibre proteins will be useful in studying the mechanism by which adenovirus infects different cell types.
Authors:
D J Von Seggern; J Kehler; R I Endo; G R Nemerow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  79 ( Pt 6)     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-09     Completed Date:  1998-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1461-8     Citation Subset:  IM    
Affiliation:
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*,  physiology
Amino Acid Sequence
Capsid / genetics*,  physiology
Capsid Proteins*
Cell Line
Gene Expression
Genetic Complementation Test
Humans
Molecular Sequence Data
Recombinant Fusion Proteins / genetics
Sequence Homology, Amino Acid
Virus Assembly
Grant Support
ID/Acronym/Agency:
EY11431/EY/NEI NIH HHS; HL54352/HL/NHLBI NIH HHS; T32 AI07354/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Recombinant Fusion Proteins; 0/hexon capsid protein, Adenovirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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