| Complementation of a fibre mutant adenovirus by packaging cell lines stably expressing the adenovirus type 5 fibre protein. | |
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MedLine Citation:
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PMID: 9634089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adenovirus-based gene therapy vectors now in use cannot be targeted to specific cell types in vivo and are immunogenic, properties which limit their clinical utility. Improved vectors lacking the genes for viral structural proteins may overcome these limitations. We have developed cell lines which stably express the adenovirus type 5 (Ad5) fibre protein in its native trimeric form. These cells can complement an Ad5 mutant with a defect in the fibre gene, and are capable of incorporating the Ad5 fibre into particles of a different Ad serotype. As the fibre protein is responsible for the initial binding of virus to cells, packaging cell lines expressing different or modified fibre proteins will be useful in studying the mechanism by which adenovirus infects different cell types. |
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Authors:
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D J Von Seggern; J Kehler; R I Endo; G R Nemerow |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of general virology Volume: 79 ( Pt 6) ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-07-09 Completed Date: 1998-07-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1461-8 Citation Subset: IM |
Affiliation:
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Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics*,
physiology Amino Acid Sequence Capsid / genetics*, physiology Capsid Proteins* Cell Line Gene Expression Genetic Complementation Test Humans Molecular Sequence Data Recombinant Fusion Proteins / genetics Sequence Homology, Amino Acid Virus Assembly |
| Grant Support | |
ID/Acronym/Agency:
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EY11431/EY/NEI NIH HHS; HL54352/HL/NHLBI NIH HHS; T32 AI07354/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Capsid Proteins; 0/Recombinant Fusion Proteins; 0/hexon capsid protein, Adenovirus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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