Document Detail

Complementary expression and repulsive signaling suggest that EphB receptors and ephrin-B ligands control cell positioning in the gastric epithelium.
MedLine Citation:
PMID:  21959989     Owner:  NLM     Status:  Publisher    
Eph receptors and ephrin ligands are membrane-bound cell-cell communication molecules with well-defined roles in development. However, their expression and functions in the gastric epithelium are virtually unknown. We detected several EphB receptors and ephrin-Bs in the gastric corpus mucosa of the adult rodent stomach by RT-PCR amplification. Immunostaining showed complementary expression patterns, with EphB receptors preferentially expressed in the deeper regions and ephrin-Bs in the superficial regions of the gastric units. EphB1, EphB2 and EphB3 are expressed in mucous neck, chief and parietal cells, respectively. In contrast, ephrin-B1 is in pit cells and proliferating cells of the isthmus. In a mouse ulcer model, EphB2 expression was upregulated in the regenerating epithelium and expanded into the isthmus. Thus, EphB/ephrin-B signaling likely occurs preferentially in the isthmus, where receptor-ligand overlap is highest. We show that EphB signaling in primary gastric epithelial cells promotes cell retraction and repulsion at least in part through RhoA activation. Based on these findings, we propose that the EphB-positive progeny of gastric stem cells migrates from the isthmus toward the bottom of the gastric glands due to repulsive signals arising from contact with ephrin-Bs, which are preferentially expressed in the more superficial regions of the isthmus and gastric pits.
Kazushige Ogawa; Natsuki Takemoto; Maki Ishii; Elena B Pasquale; Takayuki Nakajima
Related Documents :
422669 - Genetic analysis of developmental mechanisms in hydra. vi. cellular composition of chim...
7231639 - Schwann cell multiplication in trembler mice.
11105049 - Cre recombinase expression in cerebellar purkinje cells.
14724389 - Antibody-mediated cns demyelination ii. focal spinal cord lesions induced by implantati...
20007469 - Dramatic reduction of prp c level and glycosylation in peripheral nerves following prp ...
12965229 - Olivocerebellar projections are necessary for exogenous trophic factors to delay heredo...
14635929 - Germ cell-somatic cell interactions during spermatogenesis.
20379539 - Cds or cdse decorated tio2 nanotube arrays from spray pyrolysis deposition: use in phot...
23212619 - αb-crystallin regulates oxidative stress-induced apoptosis in cardiac h9c2 cells via t...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-30
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  -     ISSN:  1432-119X     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ourai-Kita, Izumisano, Osaka, 598-8531, Japan,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Hematopoietic recovery kinetics predicts for poor CD34+ cell mobilization after cyclophosphamide che...
Next Document:  Effect of Bacoside Extract from Bacopa monniera on Physical Fatigue Induced by Forced Swimming.