Document Detail


Complementary directed modifications of nucleic acids and oncogene-directed mutagenesis in vivo.
MedLine Citation:
PMID:  1841269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High reactivity of the polyalkylating ss oligomers that were sense or antisense 30-200-mers containing sequences complementary to E1 oncogenes of simian adenovirus SA7 and one alkylating residue -CH2CH2N(C2H5OH) (CH2)3N(Ph-p-CH2OH)CH2CH2Cl per each 25 bases of oligomers was demonstrated in vitro by alkylation of ss DNA of recombinant M 13 mp8E1 and mp9E1 phages with inserted E1 sequences of adenovirus oncogene and then by followed complete and selective elimination of E1 sequences from recombinant ss DNA. Treatment of rodent cell cultures transformed by oncogenic SA7 with polyalkylating oligomers which are complementary to the long region of the minus or plus chains of E1 DNA of SA7 revealed a rather high extent of mutant cell clones formation. The cells formed were normalized; they had lost some properties of the transformed cells. Dividing cell clones inherited the new phenotypic properties: morphology, slower and more limited proliferation, and higher dependence on bovine serum growth factors. Some of the mutant cell DNAs demonstrated different mutations in the E1A sequences of the integrated proviral oncogene. There were exchanges G to C (leu to val) in the 525 and C to A (asp to tyr) in the 555 positions of E1A oncogene. Besides a deletions in the 1057-1477 E1A region or/and a mutation in the 1457-1477 of E1A were observed. Thus the inherited cell normalization observed is performed due to oncogene-directed mutagenesis in vivo.
Authors:
N Grineva; N Satz; V Pantin; G Solojev; V Surin; A Krutov; T Borovikova; E Shukova
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids symposium series     Volume:  -     ISSN:  0261-3166     ISO Abbreviation:  Nucleic Acids Symp. Ser.     Publication Date:  1991  
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8007206     Medline TA:  Nucleic Acids Symp Ser     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  143-6     Citation Subset:  IM    
Affiliation:
Institute for Experimental Hematology and Biotechnology, Research Center for Hematology, Moscow, USSR.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics
Adenovirus E1 Proteins / genetics
Alkylation
Animals
Cell Line, Transformed
Mutagenesis*
Oligonucleotides / chemical synthesis*,  pharmacology*
Oligonucleotides, Antisense / chemical synthesis*,  pharmacology*
Oncogenes / drug effects*
Rats
Chemical
Reg. No./Substance:
0/Adenovirus E1 Proteins; 0/Oligonucleotides; 0/Oligonucleotides, Antisense

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