Document Detail

Complementarity-determining region 3 spectratyping analysis of the TCR repertoire in multiple sclerosis.
MedLine Citation:
PMID:  12707368     Owner:  NLM     Status:  MEDLINE    
Multiple sclerosis (MS) is considered to be an autoimmune disease mediated by T cells reactive with Ags in the CNS. Therefore, it has been postulated that neuroantigen-reactive T cells bearing particular types of TCRs are expanded clonally during the course of the disease. However, there is a controversy with regard to the TCR usage by T cells associated with the development of MS. By the use of complementarity-determining region 3 spectratyping analysis that is shown to be a useful tool for identification of pathogenic TCR in autoimmune disease models, we tried to demonstrate that spectratype was T cells bearing particular types of TCR are activated in MS patients. Consequently, it was found that Vbeta5.2 were often oligoclonally expanded in peripheral blood of MS patients, but not of healthy subjects. Sequence analysis of the complementarity-determining region 3 region of spectratype-derived TCR clones revealed that the predominant TCR clone was different from patient to patient, but that similar results were obtained in a patient examined at different time points. More importantly, examination of cerebrospinal fluid T cells and longitudinal studies of PBLs from selected patients revealed that Vbeta5.2 expansion was detectable in the majority of patients examined. These findings suggest that Vbeta5.2 spectratype expansion is associated with the development of MS and that TCR-based immunotherapy can be applicable to MS patients if the TCR activation pattern of each patient is determined at different stages of the disease.
Yoh Matsumoto; Wong Kee Yoon; Youngheun Jee; Kazuo Fujihara; Tatsuro Misu; Shigeru Sato; Ichiro Nakashima; Yasuto Itoyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  170     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4846-53     Citation Subset:  AIM; IM    
Department of Molecular Neuropathology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo, Japan.
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MeSH Terms
Amino Acid Sequence
Cloning, Molecular
Complementarity Determining Regions / blood,  cerebrospinal fluid,  genetics*,  isolation & purification*
Cross-Sectional Studies
HLA-DR Antigens / blood,  genetics
Histocompatibility Testing / methods
Longitudinal Studies
Lymphocyte Subsets / chemistry,  immunology
Middle Aged
Molecular Sequence Data
Multiple Sclerosis / blood,  cerebrospinal fluid,  genetics*,  immunology*
Multiple Sclerosis, Chronic Progressive / blood,  cerebrospinal fluid,  genetics,  immunology
Multiple Sclerosis, Relapsing-Remitting / blood,  cerebrospinal fluid,  genetics,  immunology
Polymerase Chain Reaction / methods
Receptors, Antigen, T-Cell, alpha-beta / blood,  genetics*,  isolation & purification*
Sequence Alignment
Reg. No./Substance:
0/Complementarity Determining Regions; 0/HLA-DR Antigens; 0/Receptors, Antigen, T-Cell, alpha-beta; 0/T cell receptor peptide Vbeta5.2; 128338-86-3/HLA-DRB1 antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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