| Complement-mediated lysis of K562 human leukemic cells by antibodies to phosphotyrosine and identification of cell surface proteins phosphorylated on tyrosine. | |
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MedLine Citation:
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PMID: 2421879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intact K562 human leukemic cells showed bright membrane immunofluorescence after staining with monoclonal antibody to O-phosphotyrosine (PTyr). Up to 60% of the cells were lysed with mouse, rabbit, or human antibodies to PTyr by a complement-mediated mechanism. A new method has been developed for identifying proteins that have PTyr residues on the outside of cell membrane, and at least two species of PTyr-containing proteins with the molecular weights of 45,000 and 36,000 were identified as the most probable candidates of the antigens responsible for the membrane fluorescence and cell lysis. |
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Authors:
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R Ogawa; M Ohtsuka; Y Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 46 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-05-23 Completed Date: 1986-05-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2507-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Cell Line Cell Membrane / immunology Complement System Proteins / immunology Cytotoxicity, Immunologic Fluorescent Antibody Technique Humans Leukemia / immunology* Membrane Proteins / immunology Molecular Weight Neoplasm Proteins / immunology* Phosphoproteins / immunology* Phosphotyrosine Tyrosine / analogs & derivatives*, immunology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/Phosphoproteins; 21820-51-9/Phosphotyrosine; 55520-40-6/Tyrosine; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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