Document Detail

Complement-binding proteins are strongly expressed by human preimplantation blastocysts and cumulus cells as well as gametes.
MedLine Citation:
PMID:  9238658     Owner:  NLM     Status:  MEDLINE    
Human preimplantation embryos, gametes and cumulus cells were studied for expression of the complement-binding proteins CD46 (membrane cofactor protein), CD55 (decay accelerating factor) and CD59 (membrane attack complex inhibitory factor) as well as complement receptors type 1 (CRI), type 2 (CR2) and type 3 (CR3). Both the CD55 and CD59 glycosyl phosphatidylinositol (GPI)-anchored proteins were expressed by the plasma membrane and zona pellucida of oocytes, early embryos and expanded preimplantation blastocysts; in contrast, CD46 was expressed only on the plasma membrane. Cumulus cells consistently expressed CD46 and, most strongly, CD59 whereas CD55 expression was variable. Monoclonal antibodies (mAbs) to CRI, CR2 and CR3 epitopes gave only occasional reactivity on oocytes and were unreactive with blastocysts, spermatozoa and cumulus cells. CD46 is expressed only on the spermatozoal inner acrosomal membrane, and CD59 on the plasma membrane; CD55 expression was confirmed on the plasma membrane as well as the inner acrosomal membrane. Control mAbs specific for factor H were usually unreactive with gametes, blastocysts and cumulus cells. These data support the concept that gametes and early embryonic cells are protected from complement-mediated attack by expression of CD46, CD55 and CD59, although these complement-binding proteins may have additional roles in reproductive events.
C T Taylor; P M Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  2     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1997-09-11     Completed Date:  1997-09-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  52-9     Citation Subset:  IM    
Reproductive Medicine Unit, Liverpool Women's Hospital, UK.
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MeSH Terms
Acrosome / metabolism
Antibodies, Monoclonal
Antigens, CD / analysis,  biosynthesis
Antigens, CD46
Antigens, CD55 / analysis,  biosynthesis
Antigens, CD59 / analysis,  biosynthesis
Blastocyst / metabolism*
Glycosylphosphatidylinositols / analysis,  biosynthesis
Immunoenzyme Techniques
Membrane Glycoproteins / analysis,  biosynthesis*
Oocytes / metabolism*
Receptors, Complement / analysis,  biosynthesis*
Receptors, Complement 3d / analysis,  biosynthesis
Sperm-Ovum Interactions
Spermatozoa / metabolism*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, CD46; 0/Antigens, CD55; 0/Antigens, CD59; 0/CD46 protein, human; 0/Glycosylphosphatidylinositols; 0/Membrane Glycoproteins; 0/Receptors, Complement; 0/Receptors, Complement 3d

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