| Complement activation by heat-killed human kidney cells: formation, activity, and stabilization of cell-bound C3 convertases. | |
| | |
MedLine Citation:
|
PMID: 6376629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Heat-killed kidney cells (HKKC) incubated with serum or purified C3 and factors B and D stain intensely for C3 by immunofluorescence. Binding of complement (C) did not occur if ethylenediaminetetraacetic acid (EDTA) was added to the C source or if factor B or D was omitted from the mixture of alternative pathway components. When the C-treated cells were washed, then incubated in purified C3 containing 10 mM EDTA, C3 conversion ensued, confirming the presence of a cell-bound C3 convertase. C3-reactive cells were not generated if EDTA was present during formation of the convertase. The addition of C3 nephritic factor (C3NeF) during formation of the C3 convertase from alternative pathway components greatly enhanced subsequent C3 cleavage, presumably by stabilizing labile C3bBb sites; however, binding of C3NeF did not appear to extend the half-life of the convertase, which was 35 min at 37 degrees C in each case. In contrast, little or no effect on C3 conversion could be detected after the addition of equivalent C3NeF to serum and HKKC. Thus, C activation by HKKC involves formation and activity of a cell-bound C3 convertase, which under certain circumstances can be stabilized by C3NeF. |
| | |
Authors:
|
P J Baker; S Adler; Y Yang; W G Couser |
Related Documents
:
|
10679229 - Enhancement of the migration of metastatic human breast cancer cells by phosphatidic acid. 3104349 - Distinct localizations of urokinase-type plasminogen activator and its type 1 inhibitor... 293739 - Analysis of genetic complementation by whole-cell microtechniques in fibroblast heterok... 10861399 - Limitations to the amplification and stability of human tissue-type plasminogen activat... 312899 - Isolation and immunological characterization of a major surface glycoprotein (gp54) pre... 8902999 - Characterization of cellular binding sites and interactive regions within reactants req... 330879 - Genetic and physiological control of host cell lysis by bacteriophage lambda. 1472869 - Elements of the rabbit uteroglobin promoter mediating its transcription in epithelial c... 23276719 - The role of necl-5 in the invasive activity of lung adenocarcinoma. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 133 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1984 Aug |
Date Detail:
|
Created Date: 1984-08-20 Completed Date: 1984-08-20 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 877-81 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cells, Cultured Complement Activating Enzymes / biosynthesis* Complement Activation* Complement C3 Nephritic Factor / metabolism Complement C3-C5 Convertases / biosynthesis*, metabolism Complement Pathway, Alternative* Hot Temperature* Humans Kidney / cytology*, enzymology, metabolism Kinetics Macrophage-1 Antigen Receptors, Complement / analysis |
| Grant Support | |
ID/Acronym/Agency:
|
AM 32082/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Complement C3 Nephritic Factor; 0/Macrophage-1 Antigen; 0/Receptors, Complement; EC 3.-/Complement Activating Enzymes; EC 3.4.21.-/Complement C3-C5 Convertases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues ...
Next Document: Antibody-independent C1 activation by E. coli.