Document Detail


Complement and neutrophil function changes after liver resection in humans.
MedLine Citation:
PMID:  19789912     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Complement activation contributes to the regulation of liver regeneration after liver resection (LR) in mice. METHODS: We hypothesized that complement activation and changes in C5a-receptors (C5aR, C5L2) on polymorphonuclear cells (PMN) and monocytes are important in clinical LR. Anaphylatoxin and C5b9 plasma levels were measured (bead-array, ELISA) (25 patients) and receptor expression was assessed after LR (19 patients) (FACS). In vitro PMN C5a-dependent chemotactic response (7 patients) as well as L-selectin shedding and Mac-1 expression (3 patients) was determined. RESULTS: C3a increased after LR (31.1 +/- 4 before LR vs. 41.6 +/- 5 ng/ml, 30 min after LR, P < 0.01), as did C5b9 (12.7 +/- 1 before LR vs. 26.9 +/- 3 ng/ml, 60 min after LR, P < 0.001). C4a and C5a decreased after LR, by 25% 24 h after LR and 30% 2 h after LR, respectively (P < 0.01). C5L2 expression decreased at 4 h, rising at 24 h after LR (PMN: 6.3 +/- 1 before LR, 3.1 +/- 1, 4 h, 8.3 +/- 2, 24 h; P < 0.01). The receptor-related changes accompanied a diminished C5a-dependent chemotactic response by PMN (42.1 +/- 17 before LR vs. 2.1 +/- 3 4 h after LR; P < 0.01) and a reduction of activation upon C5a-R stimulation as measured by L-selectin shedding and Mac-1 expression on PMN. Changes in C5L2 expression on monocytes paralleled postoperative impairment of liver function. CONCLUSIONS: These results indicate that complement components are released after clinical LR and subsequently PMN display altered C5a-dependent functional responses.
Authors:
Christoph Werner Strey; Britta Siegmund; Saskia Rosenblum; Rosa Maria Marquez-Pinilla; Elsie Oppermann; Markus Huber-Lang; John D Lambris; Wolf O Bechstein
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  World journal of surgery     Volume:  33     ISSN:  1432-2323     ISO Abbreviation:  World J Surg     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-03-18     Completed Date:  2010-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704052     Medline TA:  World J Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2635-43     Citation Subset:  IM    
Affiliation:
Department of General and Vascular Surgery, Medical School, Johann Wolfgang Goethe-University Frankfurt, Theodor-Stern-Kai 7, Frankfurt am Main 60590, Germany. strey@gmx.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Anaphylatoxins / immunology
Animals
Complement System Proteins / immunology*
Hepatectomy
Humans
Liver / immunology*
Liver Regeneration / immunology*
Male
Mice
Middle Aged
Monocytes / immunology
Neutrophils / immunology*
Grant Support
ID/Acronym/Agency:
DK-59422/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Anaphylatoxins; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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