Document Detail

Competitive dose-modification between ascorbate and misonidazole in human and hamster cells: effects of glutathione depletion.
MedLine Citation:
PMID:  2902158     Owner:  NLM     Status:  MEDLINE    
Depletion of glutathione by pretreatment with buthionine sulphoximine greatly enhances the radiosensitizing efficiency of misonidazole in mammalian cells in vitro, but a similar effect has not yet been observed in vivo. In thiol-depleted V79 Chinese hamster cells and human HT1080 fibrosarcoma cells, physiological concentrations of ascorbate greatly reduce misonidazole radiosensitization, although there is little effect of ascorbate on misonidazole sensitization in untreated cells. The effect of ascorbate on misonidazole radiosensitization is not markedly dependent on the extracellular concentration of ascorbate; this may be explained by the non-equilibrium uptake of ascorbate at different extracellular concentrations. Failure to obtain a large enhancement of misonidazole radiosensitization as a result of buthionine sulphoximine treatment in vivo may be due, in part at least, to the presence of ascorbate.
R J Hodgkiss; M R Stratford
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  54     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-09     Completed Date:  1988-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  601-10     Citation Subset:  IM; S    
Gray Laboratory of the Cancer Research Campaign, Mount Vernon Hospital, Northwood, Middlesex, U.K.
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MeSH Terms
Ascorbic Acid / pharmacology*
Cell Line
Cell Survival / drug effects,  radiation effects
Glutathione / metabolism*
Misonidazole / antagonists & inhibitors*
Radiation Tolerance*
Tumor Cells, Cultured
Reg. No./Substance:
13551-87-6/Misonidazole; 50-81-7/Ascorbic Acid; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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