| Competitive capacity of HIV-1 strains carrying M184I or Y181I drug-resistant mutations. | |
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MedLine Citation:
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PMID: 19493444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Virus with nucleoside reverse transcriptase inhibitors (NRTIs) or nonnucleoside reverse transcriptase inhibitors (NNRTIs) resistant mutations show different evolution tendencies when the anti-viral therapies are interrupted. Understanding the replication fitness of drug-resistant virus is important for the study of the prevalence of drug-resistance. For this purpose, we characterized the replication capacity of HIV-1 virus carrying lamivudine (3TC) or nevirapine (NVP) resistant mutations. METHODS: 3TC and NVP resistant variants were induced in vitro by selecting wild type virus in the presence of drugs. For the competitive replication assay, drug-resistant variants were cocultured with wild-type virus in the presence or absence of drugs. The ratios of the viral species were determined over time by using a real-time RT-PCR-based assay. RESULTS: 3TC-resistant (M184I mutation) and NVP-resistant (Y181I mutation) virus should be selected in vitro in two different ways. The competitive replication assay showed that the ratio of virus carrying a M184I mutation increased from 98.8%, while the wild type virus decreased to 1.2% after 4 passages in the presence of 3TC; the percentage of virus carrying the Y181I mutation increased to 90.5%, while wild type virus decreased to 9.5% in the presence of NVP. In the absence of drugs, the ratio of virus carrying the M184I mutation decreased to 5.3%, while wild type virus increased to 94.7%; the ratio of virus carrying Y181I increased to 75%, while wild type virus decreased to 25% after 4 passages. CONCLUSIONS: The NVP-resistant virus is fitter than wild type virus even in the absence of NVP that may be the reason that NNRTIs-resistant virus is spreading quickly. |
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Authors:
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Jue Li; Lin Li; Han-ping Li; Dao-min Zhuang; Si-yang Liu; Yong-jian Liu; Zuo-yi Bao; Zheng Wang; Jing-yun Li |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chinese medical journal Volume: 122 ISSN: 0366-6999 ISO Abbreviation: Chin. Med. J. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-06-04 Completed Date: 2009-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7513795 Medline TA: Chin Med J (Engl) Country: China |
Other Details:
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Languages: eng Pagination: 1081-6 Citation Subset: IM |
Affiliation:
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Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Drug Resistance, Viral / genetics* HIV-1 / drug effects, genetics, growth & development, physiology* Humans Lamivudine / pharmacology Mutation Nevirapine / pharmacology Reverse Transcriptase Inhibitors / pharmacology Reverse Transcriptase Polymerase Chain Reaction Virus Replication / genetics, physiology |
| Chemical | |
Reg. No./Substance:
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0/Reverse Transcriptase Inhibitors; 129618-40-2/Nevirapine; 134678-17-4/Lamivudine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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