Document Detail


Competition between benzo[a]pyrene and various steroids for cytochrome P-450-dependent rat liver monooxygenases.
MedLine Citation:
PMID:  6450647     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytochrome P-450-dependent monooxygenases are able to oxidize a large variety of endogenous and exogenous substrates. This paper describes the in vitro interaction between benzopyrene and steroids at the level of two rat liver monooxygenases: steroid-16 alpha-hydroxylase and aryl hydrocarbon hydroxylase (AHH). The results obtained suggest the following conclusions: (1) Steroid-16 alpha-hydroxylase is partially supported by a specific cytochrome P-450 form which is not inhibited in vitro by exogenous substrates. Steroid-16 alpha-hydroxylase is completely independent from cytochrome P1-450 (or P-448), as it is insensitive, in vitro, to alpha-naphthoflavone; (2) AHH is supported by two cytochrome P-450 forms: a specific form which is inducible by methylcholanthrene and inhibited in vitro by alpha-naphthoflavone, but is insensitive to metyrapone and steroids; and another less specific form which is inhibited by metyrapone and steroids in vitro.
Authors:
F Pasleau; P Kremers; J E Gielen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemico-biological interactions     Volume:  34     ISSN:  0009-2797     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  1981 Mar 
Date Detail:
Created Date:  1981-04-24     Completed Date:  1981-04-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  279-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aryl Hydrocarbon Hydroxylases / antagonists & inhibitors*
Benzoflavones / pharmacology
Benzopyrene Hydroxylase / antagonists & inhibitors*,  metabolism
Benzopyrenes / pharmacology*
Binding, Competitive
Cytochrome P-450 Enzyme System / metabolism*
Dehydroepiandrosterone / pharmacology
Enzyme Induction
Female
Liver / drug effects
Male
Methylcholanthrene / pharmacology
Metyrapone / pharmacology
Microsomes, Liver / enzymology
Phenobarbital / pharmacology
Pregnanolone / pharmacology
Progesterone / pharmacology
Rats
Steroid 16-alpha-Hydroxylase*
Steroid Hydroxylases / analysis*,  metabolism
Steroids / pharmacology*
Substrate Specificity
Testosterone / pharmacology
Chemical
Reg. No./Substance:
0/Benzoflavones; 0/Benzopyrenes; 0/Steroids; 128-20-1/Pregnanolone; 50-06-6/Phenobarbital; 53-43-0/Dehydroepiandrosterone; 54-36-4/Metyrapone; 56-49-5/Methylcholanthrene; 57-83-0/Progesterone; 58-22-0/Testosterone; 604-59-1/alpha-naphthoflavone; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/Steroid Hydroxylases; EC 1.14.14.-/Benzopyrene Hydroxylase; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Steroid 16-alpha-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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