| Compensatory phospholipid digestion is required for cholesterol absorption in pancreatic phospholipase A(2)-deficient mice. | |
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MedLine Citation:
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PMID: 11266383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIMS: Numerous studies have suggested phospholipid inhibition of dietary cholesterol absorption through the gastrointestinal tract. This study addressed the importance of luminal phospholipid hydrolysis in this process. METHODS: The effect of phospholipase inhibition on cholesterol transport from intestinal lumen to the lymphatics was evaluated in lymph fistula rats. Cholesterol and phospholipid absorption efficiency in intact animals was evaluated in control and phospholipase A(2) (PLA2) gene-targeted mice. RESULTS: The PLA2 inhibitor FPL 67047XX retarded cholesterol absorption in a lymph fistula rat model. Under basal chow-fed dietary conditions, cholesterol absorption efficiency from a single bolus meal, and plasma lipid levels, were similar among PLA2+/+, PLA2+/-, and PLA2-/- mice. Interestingly, the nonhydrolyzable phospholipid dioleoyl ether phosphatidylcholine suppressed cholesterol absorption by 10% to 18% in mice without regard to their PLA2 genotype. When 1-palmitoyl-2-[(14)C]oleoyl-phosphatidylcholine was used as the substrate, the radiolabeled phospholipid was found to be hydrolyzed and absorbed with equal efficiency in PLA2+/+, PLA2+/-, and PLA2-/- mice. CONCLUSIONS: These results suggested that although phospholipid digestion in the intestinal lumen is a prerequisite for efficient cholesterol absorption, additional enzyme(s) can compensate for pancreatic PLA2 in catalyzing phospholipid digestion and facilitating cholesterol absorption in PLA2 knockout mice. |
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Authors:
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B L Richmond; A C Boileau; S Zheng; K W Huggins; N A Granholm; P Tso; D Y Hui |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 120 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-03-27 Completed Date: 2001-04-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 1193-202 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio 45267-0529, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Radioisotopes / diagnostic use Cholesterol, Dietary / pharmacokinetics* Digestive System Fistula / metabolism Female Intestinal Absorption / physiology* Lymph / metabolism Male Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Knockout Phospholipases A / genetics*, metabolism Phospholipases A2 Pregnancy Rats Rats, Sprague-Dawley Triglycerides / pharmacokinetics |
| Grant Support | |
ID/Acronym/Agency:
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DK54504/DK/NIDDK NIH HHS; F32 DK10065/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Cholesterol, Dietary; 0/Triglycerides; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
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