Document Detail

Compatibility of superparamagnetic iron oxide nanoparticle labeling for ¹H MRI cell tracking with ³¹P MRS for bioenergetic measurements.
MedLine Citation:
PMID:  20853523     Owner:  NLM     Status:  MEDLINE    
Labeling of cells with superparamagnetic iron oxide nanoparticles permits cell tracking by (1)H MRI while (31)P MRS allows non-invasive evaluation of cellular bioenergetics. We evaluated the compatibility of these two techniques by obtaining (31)P NMR spectra of iron-labeled and unlabeled immobilized C2C12 myoblast cells in vitro. Broadened but usable (31)P spectra were obtained and peak area ratios of resonances corresponding to intracellular metabolites showed no significant differences between labeled and unlabeled cell populations. We conclude that (31)P NMR spectra can be obtained from cells labeled with sufficient iron to permit visualization by (1)H imaging protocols and that these spectra have sufficient quality to be used to assess metabolic status. This result introduces the possibility of using localized (31)P MRS to evaluate the viability of iron-labeled therapeutic cells as well as surrounding host tissue in vivo.
Zhuoli Zhang; Brynne Hancock; Stephanie Leen; Sharan Ramaswamy; Steven J Sollott; Kenneth R Boheler; Magdalena Juhaszova; Edward G Lakatta; Richard G Spencer; Kenneth W Fishbein
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2010-09-20
Journal Detail:
Title:  NMR in biomedicine     Volume:  23     ISSN:  1099-1492     ISO Abbreviation:  NMR Biomed     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-16     Completed Date:  2011-06-10     Revised Date:  2011-08-29    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  England    
Other Details:
Languages:  eng     Pagination:  1166-72     Citation Subset:  IM    
Copyright Information:
Published in 2010 by John Wiley & Sons, Ltd.
Laboratory of Cardiovascular Science, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.
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MeSH Terms
Cell Differentiation
Cell Proliferation
Cell Separation
Cell Survival
Cell Tracking / methods*
Energy Metabolism / physiology*
Ferric Compounds / chemistry*
Magnetic Resonance Imaging / methods*
Magnetic Resonance Spectroscopy
Magnetite Nanoparticles / chemistry*
Myoblasts / cytology*,  metabolism
Phosphorus Isotopes
Staining and Labeling*
Reg. No./Substance:
0/Ferric Compounds; 0/Magnetite Nanoparticles; 0/Phosphorus Isotopes; 0/Protons; 1309-37-1/ferric oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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