Document Detail


Compatibility of lyotropic liquid crystals with viruses and mammalian cells that support the replication of viruses.
MedLine Citation:
PMID:  15955554     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report a study that investigates the biocompatibility of materials that form lyotropic liquid crystals (LCs) with viruses and mammalian cells that support the replication of viruses. This study is focused on aqueous solutions of tetradecyldimethyl-amineoxide (C(14)AO) and decanol (D), or disodium cromoglycate (DSCG; C(23)H(14)O(11)Na(2)), which can form optically birefringent, liquid crystalline phases. The influence of these materials on the ability of vesicular stomatitis virus (VSV) to infect human epitheloid cervical carcinoma (HeLa) cells was examined by two approaches. First, VSV was dispersed in aqueous C(14)AO+ D or DSCG, and then HeLa cells were inoculated by contacting the cells with the aqueous C(14)AO + D or DSCG containing VSV. The infectivity of VSV to the HeLa cells was subsequently determined. Second, VSV was incubated in LC phases of either C(14)AO + D or DSCG for 4 h, and the concentration (titer) of infectious virus in the LC was determined by dilution into cell culture medium and subsequent inoculation of HeLa cells. Using these approaches, we found that the LC containing C(14)AO + D caused inactivation of virus as well as cell death. In contrast, we determined that VSV retained its infectivity in the presence of aqueous DSCG, and that greater than 74-82% of the HeLa cells survived contact with aqueous DSCG (depending on concentration of DSCG). Because VSV maintained its function (and we infer structure) in LCs formed from DSCG, we further explored the influence of the virus on the ordering of the LC. Whereas the LC formed from DSCG was uniformly aligned on surfaces prepared from self-assembled monolayers (SAMs) of HS(CH(2))(11)(OCH(2)CH(2))(4)OH on obliquely deposited films of gold in the absence of VSV, the introduction of 10(7)-10(8) infectious virus particles per milliliter caused the LC to assume a non-uniform orientation and a colorful appearance that was readily distinguished from the uniformly aligned LCs. Control experiments using cell lysates with equivalent protein concentrations but no virus did not perturb the uniform alignment of the LC.
Authors:
Li-Lin Cheng; Yan-Yeung Luk; Christopher J Murphy; Barbara A Israel; Nicholas L Abbott
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-08-02     Completed Date:  2005-12-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  7173-82     Citation Subset:  IM    
Affiliation:
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials / chemistry*
Crystallization / methods*
Hela Cells
Humans
Materials Testing
Solutions
Vesicular stomatitis Indiana virus / growth & development*,  ultrastructure*
Virus Cultivation / methods*
Virus Replication / physiology*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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