Document Detail


Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses.
MedLine Citation:
PMID:  17622614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycoprotein gH, together with its chaperone gL and a third glycoprotein gB, is essential for cell-cell fusion and virus-cell fusion mediated by herpesviruses. Epstein-Barr virus (EBV), the prototype human lymphocryptovirus, requires a fourth glycoprotein gp42 to support fusion with B cells in addition to epithelial cells. Two other lymphocryptoviruses, the rhesus lymphocryptovirus (Rh-LCV) and the common marmoset lymphocryptovirus (CalHV3), have been sequenced in their entirety and each has a gp42 homologue. Combinations of proteins from EBV, Rh-LCV and CalHV3 were able to mediate fusion of epithelial cells, but, even when complexed with EBV gp42, only Rh-LCV and not CalHV3 proteins were able to mediate fusion with human B cells. CalHV3 gL was also unable to function effectively as a chaperone for EBV or Rh-LCV gH. The Rh-LCV gH homologue supported more fusion than EBV gH with an epithelial cell and supported the highest levels of fusion with a B cell. Chimeric constructs made from Rh-LCV gH and EBV gH that have 85.4 % sequence identity should prove useful for mapping the regions of gH that are of importance to fusion as a whole and to B-cell fusion in particular.
Authors:
Liguo Wu; Lindsey M Hutt-Fletcher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of general virology     Volume:  88     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-09-07     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2129-36     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / physiology,  virology
Cell Fusion
Cell Line
Epithelial Cells / physiology,  virology
Glycoproteins / metabolism*
Herpesvirus 4, Human / chemistry,  physiology
Humans
Lymphocryptovirus / chemistry,  physiology*
Viral Envelope Proteins / metabolism*
Viral Proteins / metabolism*
Virus Attachment
Grant Support
ID/Acronym/Agency:
AI20662/AI/NIAID NIH HHS; DE016669/DE/NIDCR NIH HHS; P20-RR018724/RR/NCRR NIH HHS; R01 AI020662/AI/NIAID NIH HHS; R01 AI020662-22/AI/NIAID NIH HHS; R01 DE016669-01/DE/NIDCR NIH HHS; R01 DE016669-07/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/BZLF2 protein, Herpesvirus 4, Human; 0/Glycoproteins; 0/Viral Envelope Proteins; 0/Viral Proteins; 0/glycoprotein H, Herpesvirus 4
Comments/Corrections

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