| Compatibility of the gH homologues of Epstein-Barr virus and related lymphocryptoviruses. | |
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MedLine Citation:
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PMID: 17622614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycoprotein gH, together with its chaperone gL and a third glycoprotein gB, is essential for cell-cell fusion and virus-cell fusion mediated by herpesviruses. Epstein-Barr virus (EBV), the prototype human lymphocryptovirus, requires a fourth glycoprotein gp42 to support fusion with B cells in addition to epithelial cells. Two other lymphocryptoviruses, the rhesus lymphocryptovirus (Rh-LCV) and the common marmoset lymphocryptovirus (CalHV3), have been sequenced in their entirety and each has a gp42 homologue. Combinations of proteins from EBV, Rh-LCV and CalHV3 were able to mediate fusion of epithelial cells, but, even when complexed with EBV gp42, only Rh-LCV and not CalHV3 proteins were able to mediate fusion with human B cells. CalHV3 gL was also unable to function effectively as a chaperone for EBV or Rh-LCV gH. The Rh-LCV gH homologue supported more fusion than EBV gH with an epithelial cell and supported the highest levels of fusion with a B cell. Chimeric constructs made from Rh-LCV gH and EBV gH that have 85.4 % sequence identity should prove useful for mapping the regions of gH that are of importance to fusion as a whole and to B-cell fusion in particular. |
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Authors:
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Liguo Wu; Lindsey M Hutt-Fletcher |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of general virology Volume: 88 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-11 Completed Date: 2007-09-07 Revised Date: 2013-06-06 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 2129-36 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / physiology, virology Cell Fusion Cell Line Epithelial Cells / physiology, virology Glycoproteins / metabolism* Herpesvirus 4, Human / chemistry, physiology Humans Lymphocryptovirus / chemistry, physiology* Viral Envelope Proteins / metabolism* Viral Proteins / metabolism* Virus Attachment |
| Grant Support | |
ID/Acronym/Agency:
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AI20662/AI/NIAID NIH HHS; DE016669/DE/NIDCR NIH HHS; P20-RR018724/RR/NCRR NIH HHS; R01 AI020662/AI/NIAID NIH HHS; R01 AI020662-22/AI/NIAID NIH HHS; R01 DE016669-01/DE/NIDCR NIH HHS; R01 DE016669-07/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/BZLF2 protein, Herpesvirus 4, Human; 0/Glycoproteins; 0/Viral Envelope Proteins; 0/Viral Proteins; 0/glycoprotein H, Herpesvirus 4 |
| Comments/Corrections | |
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