Document Detail


Compartments within the lymph node cortex of calves and adult cattle differ in the distribution of leukocyte populations: an immunohistochemical study using computer-assisted morphometric analysis.
MedLine Citation:
PMID:  9617588     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The combination of an immunohistochemical technique and a panel of monoclonal antibodies was used to investigate the presence of leukocyte populations in the distal jejunal lymph node of 3-4 week old calves and adult cattle. The application of computer-assisted morphometric analysis enabled information to be obtained on the distribution of leukocyte populations in lymphoid compartments of the lymph node cortex. Semi-quantitative estimates of the areas of staining in histological sections showed that calves possessed significantly fewer B-cells and CD4+ cells in the outer cortex and significantly fewer T-cells (CD4+, CD8+ and gamma delta T-cells) in the deep cortex. These findings were interpreted to be a possible consequence of immunosuppression resulting from the passive transfer of maternal immunity in colostrum. The presence of some B-cell follicles in the region defined as the deep cortex suggested the on-going differentiation of this predominantly T-cell compartment. The larger presence of interdigitating cells (IDC) in the deep cortex of calves than adults was suggested by significantly larger CD1+ populations and it was argued that this could be the result of the confrontation with exogenous antigen faced by calves in early postnatal life. Antigen presenting populations, pan MHC II+ and MHC II DQ+ populations, were increased in all compartments of calf lymph nodes but were not significantly different from the populations in adult lymph nodes. Variance component analysis of the data generated in the present study showed that the image analysis technique was an effective and statistically powerful approach to investigate leukocyte populations within the specific microenvironments of the lymph node.
Authors:
G Gunnes; C M Press; A Tverdal; T Landsverk
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  22     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:    1998 Jan-Feb
Date Detail:
Created Date:  1998-08-26     Completed Date:  1998-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  111-23     Citation Subset:  IM; X    
Affiliation:
Department of Morphology, Genetics and Aquatic Biology, Norwegian College of Veterinary Medicine, Oslo, Norway.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Analysis of Variance
Animals
Antigens, CD / analysis
B-Lymphocytes / cytology
CD4-Positive T-Lymphocytes / cytology
CD8-Positive T-Lymphocytes / cytology
Cattle
Histocompatibility Antigens Class II / analysis
Image Processing, Computer-Assisted
Immunohistochemistry
Jejunum*
Lymph Nodes / anatomy & histology*,  immunology*
Lymphocyte Subsets*
Receptors, Antigen, T-Cell, gamma-delta / analysis
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Histocompatibility Antigens Class II; 0/Receptors, Antigen, T-Cell, gamma-delta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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