Document Detail

Compartmentation of free amino acids for protein synthesis in rat liver.
MedLine Citation:
PMID:  4447629     Owner:  NLM     Status:  MEDLINE    
The concept that a general intracellular pool serves as the sole precursor of amino acids for protein biosynthesis has been vigorously debated in recent years. To help resolve this controversy, we followed the distribution of intraperitoneally administered [(3)H]valine in the tRNA and the extracellular and intracellular compartments of rat liver. The specific radioactivity of the valine released from isolated tRNA was 2-3 times higher than that of intracellular valine, suggesting that the intracellular pool cannot be the sole precursor of amino acids used for charging tRNA. In addition, the specific radioactivity of the tRNA was only half that of the extracellular valine. Therefore it is unlikely that the valyl-tRNA is charged exclusively with amino acids derived from the extracellular pool. A model is proposed which stipulates that both extracellular and intracellular amino acids contribute to a restricted compartment that funnels amino acids towards protein biosynthesis.
J Airhart; A Vidrich; E A Khairallah
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  140     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1974 Jun 
Date Detail:
Created Date:  1975-04-08     Completed Date:  1975-04-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  539-45     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism*
Injections, Intraperitoneal
Liver / metabolism*
Models, Biological
Protein Biosynthesis*
RNA, Transfer / metabolism*
Valine / metabolism
Reg. No./Substance:
0/Amino Acids; 10028-17-8/Tritium; 7004-03-7/Valine; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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