| Compartmental apoptosis and neutrophil accumulation in severe peritonitis. | |
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MedLine Citation:
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PMID: 20036382 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Migration and activation of polymorphonuclear neutrophils (PMN) and apoptosis are central to inflammatory tissue damage. This study examines the relation of these processes, and their expression in the abdominal, systemic, and bronchoalveolar compartments in patients with severe peritonitis. MATERIALS AND METHODS: Thirty-one consecutive patients undergoing laparotomy for severe secondary peritonitis. Eight operated patients without peritonitis and 10 long-term mechanically ventilated noninfected patients served as controls. Peritoneal fluid, blood, and bronchoalveolar lavage fluid (BALF) was obtained on d 0 (day of initial laparotomy), 2, and 3. Levels of chemokines (interleukin (IL)-8 and monocyte chemotactic protein (MCP)-1), PMN-counts, PMN activation [myeloperoxidase (MPO), elastase] and apoptosis (nucleosomes) were determined. RESULTS: In peritonitis patients, levels of chemokines and markers of PMN sequestration were increased in all compartments. IL-8 levels were higher in BALF than in plasma, and did not originate from the circulation or from lysis of alveolar cells. Pulmonary nucleosome levels were higher in patients who died (P=0.020), and corresponded with PMN-count in BALF (P<0.001), levels of chemokines (IL-8, P=0.003; MCP-1, P=0.001), and PMN-activation (MPO, P<0.001; elastase P=0.007). CONCLUSION: Severe peritonitis produces an early pulmonary expression of chemoattractants creating a gradient for PMN sequestration and activation into the lung. These parameters are associated with expression of apoptosis in the lung, which is increased in nonsurviving peritonitis patients. |
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Authors:
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J W Olivier van Till; Suzanne Q van Veen; Veronique den Broeder; Paul Bresser; Rene Lutter; Theo A Out; Marcus J Schultz; Dirk J Gouma; Marja A Boermeester |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-01 |
Journal Detail:
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Title: The Journal of surgical research Volume: 164 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2010-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 321-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Surgery, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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APACHE Abdomen / physiopathology Aged Apoptosis / physiology* Bronchoalveolar Lavage Fluid Chemokine CCL2 / blood Chemotaxis Female Humans Interleukin-8 / blood Length of Stay Leukocyte Count Male Middle Aged Neutrophils / physiology* Nucleosomes / physiology Peritonitis / blood, physiopathology*, surgery* Peroxidase / blood Positive-Pressure Respiration |
| Chemical | |
Reg. No./Substance:
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0/CCL2 protein, human; 0/Chemokine CCL2; 0/Interleukin-8; 0/Nucleosomes; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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