Document Detail

Comparison of ultrastructure of germinating pea leaves prepared by high-pressure freezing-freeze substitution and conventional chemical fixation.
MedLine Citation:
PMID:  7650449     Owner:  NLM     Status:  MEDLINE    
High-pressure freezing-freeze substitution (HPF-FS) methods were applied to germinating pea leaves. Good ultrastructural preservation without visible freezing damage was obtained up to 200 microns in thickness. Compared to conventional chemical fixation (CF), cellular membranes were smoother without undulation, and organelles appeared more turgid. The matrices of cytoplasm and organelles were denser and more homogeneous. The features imply that HPF-FS samples retain more substances and ultrastructure closer to the living state. There were differences in membrane stainability among organelles in HPF-FS specimens, which were not seen after CF. Bundles of microfilaments were observed frequently after HPF-FS.
Y Kaneko; P Walther
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of electron microscopy     Volume:  44     ISSN:  0022-0744     ISO Abbreviation:  J Electron Microsc (Tokyo)     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-09-26     Completed Date:  1995-09-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7611157     Medline TA:  J Electron Microsc (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  104-9     Citation Subset:  IM    
Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, Japan.
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MeSH Terms
Freeze Substitution / methods*
Peas / ultrastructure*
Plant Leaves / ultrastructure*
Tissue Preservation
Grant Support
Reg. No./Substance:

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