| Comparison of ultrasound-guided vs laparoscopic transvaginal ovum pick-up (OPU) in simmental heifers. | |
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MedLine Citation:
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PMID: 10734477 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transvaginal ovum pick-up (OPU) offers several advantages over standard embryo transfer procedures. For a systematic comparison of the ultrasound-guided (U-OPU) and the laparoscopic OPU (L-OPU) method, groups of Simmental heifers were subjected to 1 of the 2 OPU-methods for 8 wk (15 sessions) followed by a treatment-free interval of 11 wk and then another 8-wk period of OPU using the alternative method. Parameters that were evaluated included the number of follicles aspirated, number and morphology of recovered cumulus-oocyte complexes (COCs), and developmental capacity of oocytes after in vitro maturation (IVM) and fertilization (IVF). Blood samples were also taken from the donors twice a wk for progesterone measurement. To evaluate effects of long-term OPU on subsequent fertility of donors, all heifers (n = 14) were inseminated during the first natural estrus after the OPU experiment. The proportion of Class I oocytes was significantly (P < 0.001) higher after U-OPU than after L-OPU (38.7% vs 21.0%). Following IVM/IVF, this difference in oocyte quality was reflected by the cleavage rate (U-OPU: 58.1%; L-OPU: 52.1%; P < 0.05) and the rate of development to morulae and blastocysts (U-OPU: 27.1%; L-OPU: 13.9%; P < 0.001). Among other factors, the greater changes in vacuum pressure during L-OPU vs U-OPU might be responsible for the difference in oocyte quality. This problem may be overcome by a more flexible system for regulating the vacuum. Progesterone levels were higher during the L-OPU than the U-OPU periods. Seven donors (50%) were diagnosed pregnant by ultrasonography on Day 28 and by palpation per rectum on Day 42. |
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Authors:
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B Santl; H Wenigerkind; W Schernthaner; J Mödl; M Stojkovic; K Prelle; W Holtz; G Brem; E Wolf |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Theriogenology Volume: 50 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 1998 Jul |
Date Detail:
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Created Date: 2000-04-25 Completed Date: 2000-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 89-100 Citation Subset: IM |
Affiliation:
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Bavarian Research Center for Biology of Reproduction (BFZF), Oberschleissheim, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle* Female Fertility Laparoscopy / methods, veterinary Ovum / ultrasonography* Pregnancy Tissue and Organ Harvesting / methods, veterinary* |
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