Document Detail


Comparison of two methods for exhaled breath condensate collection.
MedLine Citation:
PMID:  16867057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Exhaled breath condensate (EBC) is a noninvasive method to obtain samples from fluids lining the respiratory surfaces. Even though various methods and devices are now available, the relative efficiency of these methods for recovering airway mediators and characterizing EBC has not been established. AIM: To compare the volume, pH, lipid mediator, and protein concentrations in EBCs collected by two commonly used commercially available devices, RTube and ECoScreen. METHODS: Exhaled breath condensates were obtained consecutively using the RTube and ECoScreen methods from 30 healthy, nonallergic adults. Samples were immediately placed on dry ice after collection. pH was measured after argon deaeration. Cysteinyl leukotrienes (cys-LTs) were measured as a representative lipid mediator and eotaxin as the protein mediator by using enzyme-linked immunosorbent assay. RESULTS: The mean volume of samples obtained with ECoScreen (1880 +/- 116 microl) was significantly higher than that obtained with RTube (1405 +/- 82 microl) (P < 0.001). Concentrations of both cys-LTs [205.4 pg/ml (65.5-472.3) with ECoScreen vs 21.6 (11.87-152.2) with RTube, P < 0.001] and eotaxin [17.0 pg/ml (11.4-22.4) with ECoScreen vs 11.7 (10.5-13.5) with RTube, P = 0.01] were significantly higher in samples collected with ECoScreen than with RTube. There was no significant difference between the pH measurements. CONCLUSION: Compared with RTube, collection of exhaled breath by ECoScreen allows larger volumes to be collected and detects protein and lipid mediators with greater sensitivity. These differences in mediator recovery may be due to the differences in the collection temperature.
Authors:
O U Soyer; E A Dizdar; O Keskin; C Lilly; O Kalayci
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Allergy     Volume:  61     ISSN:  0105-4538     ISO Abbreviation:  Allergy     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2006-12-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1016-8     Citation Subset:  IM    
Affiliation:
Pediatric Allergy and Asthma Unit, Hacettepe University School of Medicine, Hacettepe, Ankara, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adult
Breath Tests* / instrumentation,  methods
Chemokine CCL11
Chemokines, CC / analysis*
Female
Humans
Hydrogen-Ion Concentration
Leukotrienes / analysis*
Male
Specimen Handling* / instrumentation,  methods
Chemical
Reg. No./Substance:
0/CCL11 protein, human; 0/Chemokine CCL11; 0/Chemokines, CC; 0/Leukotrienes

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