| Comparison of 2 high-throughput spectral techniques to predict differences in diet composition of grazing sheep and cattle. | |
| | |
MedLine Citation:
|
PMID: 20118417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Diet composition can be estimated in free-ranging animals by the use of n-alkane and long-chain fatty alcohol concentrations in feces. However, this technique involves relatively laborious and costly analytical techniques. Two spectroscopy techniques were investigated as a way of determining whether dietary differences are likely, thus indicating whether the more expensive and labor-intensive techniques for more detailed analysis are justified. Fourier-transform infrared spectroscopy (FTIR) and front-face fluorescence emission spectroscopy (lambda(excitation) = 380 nm, lambda(emission) = 600 to 760 nm) were used to analyze fecal samples collected from 2 different breeds of cattle and sheep (4 groups in total, n = 6 per group) grazing moorland plants in 2 grazing sessions. These fecal samples were also analyzed for alkane and alcohol concentrations. Fourier-transform infrared spectra, particularly in the alkane regions, demonstrated clear separation between animal species. Fluorescence emission spectra showed similar separation; fluorophores were most likely chlorophylls and their derivatives. Multivariate analysis of all 3 data sets showed similar variation within and between groups of cattle and sheep, indicating differences in diet selection particularly between species, but also between breed and grazing session. Both spectroscopy methods showed utility in suggesting differences in diet composition that would be worth investigating using more detailed chemical analyses. Of the 2 techniques, the FTIR spectroscopy gave the better comparative results, being able to detect differences in sampling months that were detected with alkanes and alcohols that the fluorescence emission spectroscopy did not detect. |
| | |
Authors:
|
J M Moorby; M D Fraser; I Parveen; M R F Lee; J P Wold |
Related Documents
:
|
21775117 - A mediterranean-style low-glycemic-load diet increases plasma carotenoids and decreases... 11182307 - Performance of west african dwarf goats fed guinea grass-verano stylo mixture, n-fertil... 15819957 - Responsiveness to progestagen-ecg-cloprostenol treatment in goat food restricted for lo... 2272977 - Aerosolization of recombinant slpi to augment antineutrophil elastase protection of pul... 6701137 - Effect of diisopropyl 1,3-dithiolan-2-ylidenemalonate on experimental fatty livers in c... 1127467 - Riboflavin and mouse hepatic cell structure and function. mitochondrial oxidative metab... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-29 |
Journal Detail:
|
Title: Journal of animal science Volume: 88 ISSN: 1525-3163 ISO Abbreviation: J. Anim. Sci. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-15 Completed Date: 2010-07-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8003002 Medline TA: J Anim Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1905-13 Citation Subset: IM |
Affiliation:
|
Institute of Biological, Environmental and Rural Sciences, Gogerddan, Aberystwyth SY23 3EB, United Kingdom. jon.moorby@aber.ac.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Husbandry Animals Cattle / physiology* Diet / veterinary* Feces / chemistry* Feeding Behavior / physiology* Sheep / physiology* Spectrometry, Fluorescence |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphorus utilization and characterization of excreta from swine fed diets containing a variety of ...
Next Document: Stepwise Chilling - Tender Pork without Compromising Water-Holding Capacity.