Document Detail


Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system.
MedLine Citation:
PMID:  22576283     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
Authors:
Haroldo Cid da Silva Junior; Sérgio da Silva E Mouta Junior; Marcos César Lima de Mendonça; Mirian Claudia de Souza Pereira; Alanderson da Rocha Nogueira; Maria Luiza Borges de Azevedo; José Paulo Gagliardi Leite; Márcia Terezinha Baroni de Moraes
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-11
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz. Av. Brasil, 4365, Pavilhão Hélio & Peggy Pereira, Rio de Janeiro, RJ, 21040-360, Brazil, haroldo.cid@bio.fiocruz.br.
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