| Comparison of two-dimensional electrophoresis patterns of heat shock protein Hsp27 species in normal and cardiomyopathic hearts. | |
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MedLine Citation:
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PMID: 10612289 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heat shock protein Hsp27 occurs in a complex pattern in human myocardial tissue. Normal and failing explanted human heart from patients with dilated cardiomyopathy (DCM) or ischemic heart failure (IHF), respectively, were analyzed by high resolution two-dimensional electrophoresis (23x30 cm) and Hsp27 immunostaining. Twelve Hsp27 spots in DCM samples were significantly altered in intensity and ten of these were significantly changed in IHF. Four spots (h1, h2, h4, h5) in DCM samples and three spots (h2, h4, h5) in IHF at a molecular mass of 28 kDa were decreased in intensity. In this study, investigating left ventricles of human myocardium, spot h4 was only detected in normal heart samples. On the other hand, spots with a lower molecular mass of 27 kDa (h14, h15, h17, h20, h21) and 22-23 kDa (46, h47, h50) increased in intensity in failing hearts, suggesting that some form of Hsp27 degradation occurs during heart failure. |
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Authors:
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C Scheler; X P Li; J Salnikow; M J Dunn; P R Jungblut |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 20 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-01-05 Completed Date: 2000-01-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY |
Other Details:
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Languages: eng Pagination: 3623-8 Citation Subset: IM |
Affiliation:
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Institute for Biochemistry, Technical University Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cardiomyopathy, Dilated / metabolism* Electrophoresis, Gel, Two-Dimensional* Female Heat-Shock Proteins / analysis* Humans Male Middle Aged Myocardial Ischemia / metabolism* Myocardium / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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