| Comparison of two commercial ELISA systems for evaluating anti-EBNA1 IgG titers. | |
| | |
MedLine Citation:
|
PMID: 23154877 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
High IgG titers against the Epstein-Barr virus nuclear antigen, EBNA-1, have been strongly correlated with the risk of developing multiple sclerosis. ELISAs are used frequently to measure EBNA-1 titers, however concerns remain regarding the accuracy of results. Ordering absolute results into rank quintiles for analysis may be preferable. Using 120 serum samples, two commercially available ELISAs (produced by DiaSorin and VirionSerion) were compared, both in terms of absolute results and rank quintiles. The positive predictive value of the VirionSerion ELISA was 99.1% when compared to the DiaSorin ELISA, however, the negative predictive value was 64.3%. Sensitivity and specificity were acceptable at 95.5% and 90.0%, respectively. There was poor correlation between absolute results, R(2) = 0.49; and the kappa coefficient for rank quintiles was low at 0.23. Although sensitivity and specificity appear adequate, the poor negative predictive value and kappa coefficient are of major concern. Care must be taken when selecting assays for experimental use. J. Med. Virol. 85:128-131, 2012. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Ruth Dobson; Joanne Topping; Gavin Giovannoni |
Related Documents
:
|
229417 - Simultaneous ultrastructural demonstration of multiple peptides in endocrine cells by a... 9358847 - Tyramine amplification technique in routine immunohistochemistry. 9915117 - A novel monoclonal antibody that recognizes apical membrane of frog taste cells. 3220797 - Microwave-stimulated incubation in immunoelectron microscopy: a quantitative study. 22644337 - Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins (hmgb1/hmgb2)... 22089857 - Remodeling of the epitope repertoire of a candidate idiotype vaccine by targeting to ly... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of medical virology Volume: 85 ISSN: 1096-9071 ISO Abbreviation: J. Med. Virol. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2012-11-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
|
Languages: eng Pagination: 128-31 Citation Subset: IM |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University, London, UK. ruth.dobson@qmul.ac.uk. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Baseline factors and early viral response (week 4) to antiviral therapy with peginterferon and ribav...
Next Document: Shedding of polyomavirus in the saliva of immunocompetent individuals.