| Comparison of three selective media for detecting Fusarium species in foods: a collaborative study. | |
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MedLine Citation:
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PMID: 8796416 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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At the Second International Workshop on Standardisation of Methods for the Mycological Examination of Foods in Baarn, 1990, three selective media for food-borne Fusarium species were recommended: Czapek-Dox Iprodione Dichloran Agar (CZID), Dichloran Chloramphenicol Peptone agar (DCPA) and Pentachloronitrobenzene Peptone agar (PPA). Lack of sufficient data made it impossible to recommend one Fusarium selective medium. In the present study nine laboratories from seven countries compared CZID, DCPA, and PPA by analysing 10 samples of flour spiked with conidia of Fusarium avenaceum and F. verticillioides (= F. moniliforme). The colony forming units were counted. The total counts on each of the three media were within a similar range for each participant, and no significant differences between the three media were evident. However, the development of Fusarium colonies was quite different on the three media, and most collaborators found it possible to differentiate these Fusarium species by pigmentation on CZID. Pigmentation was much less conspicuous on PPA, and inconspicuous in DCPA cultures. CZID is recommended as the best currently available selective medium for Fusarium isolates from foods. |
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Authors:
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U Thrane |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International journal of food microbiology Volume: 29 ISSN: 0168-1605 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-10-24 Completed Date: 1996-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 149-56 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark. ut@ibt.dtu.dk. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminoimidazole Carboxamide
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analogs & derivatives Aniline Compounds Chloramphenicol Colony Count, Microbial Culture Media / chemistry* Food Microbiology* Fungicides, Industrial* Fusarium / isolation & purification* Hydantoins* Nitrobenzenes Peptones Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Aniline Compounds; 0/Culture Media; 0/Fungicides, Industrial; 0/Hydantoins; 0/Nitrobenzenes; 0/Peptones; 360-97-4/Aminoimidazole Carboxamide; 36734-19-7/iprodione; 56-75-7/Chloramphenicol; 82-68-8/quintozene; 99-30-9/dichloran |
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