Document Detail


Comparison of three selective isolation media for the detection of L. monocytogenes in foods.
MedLine Citation:
PMID:  1930568     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
391 different foods were examined in order to compare three selective isolation media for Listeria: blood agar with nalidixic acid, Palcam agar, Listeria selective Oxford medium (Oxford agar). The percentage of positive samples (presence of Listeria in 25 g) obtained on the three media after an enrichment procedure is identical: 15.8%. The species L. monocytogenes is found in respectively 8.43%, 8.43% and 8.18% of the foods examined on Palcam agar, Oxford agar and nalidixic acid agar; this represents more than 50% of the isolates of the genus Listeria. However, Palcam agar and Oxford agar offer the advantages of a great reduction of the development of the contaminating microflora and a clearly less fastidious reading. The technique used for the direct counting of L. monocytogenes on these two selective media does not allow the detection of a low contamination of foods (theoretical positivity threshold = 100 Listeria/g): 65.2% of the positive samples would not have been detected without prior enrichment. 41.1% of the isolated L. monocytogenes belong to serovar 1/2a, 5.8% to serovar 1/2b, 20.58% to serovar 1/2c, 5.8% to serovar 3b and 24.47% to serovar 4b.
Authors:
D Art; P Andre
Related Documents :
20085278 - Evaluation of a real-time polymerase chain reaction (pcr) assay for detection of anisak...
10874998 - Biotechnical use of polymerase chain reaction for microbiological analysis of biologica...
2494938 - Antibacterial activity of hen egg white lysozyme against listeria monocytogenes scott a...
11735298 - Sample preparation methods for pcr detection of escherichia coli o157:h7, salmonella ty...
17429918 - Swedish pre-school children eat too much junk food and sucrose.
11077928 - Isotope studies of hydrogen and oxygen in ground ice-experiences with the equilibration...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  275     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-12-24     Completed Date:  1991-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Affiliation:
Institut d'Hygiène et d'Epidémiologie, Département Microbiologie, Bruxelles, Belgique.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cheese
Colony Count, Microbial
Culture Media
Fish Products
Food Microbiology*
Listeria monocytogenes / growth & development,  isolation & purification*
Meat
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation of Klebsiella terrigena from human feces: biochemical reactions, capsule types, and antibi...
Next Document:  Susceptibilities of motile Aeromonas sp. to antimicrobial agents.