Document Detail

Comparison of three selective isolation media for the detection of L. monocytogenes in foods.
MedLine Citation:
PMID:  1930568     Owner:  NLM     Status:  MEDLINE    
391 different foods were examined in order to compare three selective isolation media for Listeria: blood agar with nalidixic acid, Palcam agar, Listeria selective Oxford medium (Oxford agar). The percentage of positive samples (presence of Listeria in 25 g) obtained on the three media after an enrichment procedure is identical: 15.8%. The species L. monocytogenes is found in respectively 8.43%, 8.43% and 8.18% of the foods examined on Palcam agar, Oxford agar and nalidixic acid agar; this represents more than 50% of the isolates of the genus Listeria. However, Palcam agar and Oxford agar offer the advantages of a great reduction of the development of the contaminating microflora and a clearly less fastidious reading. The technique used for the direct counting of L. monocytogenes on these two selective media does not allow the detection of a low contamination of foods (theoretical positivity threshold = 100 Listeria/g): 65.2% of the positive samples would not have been detected without prior enrichment. 41.1% of the isolated L. monocytogenes belong to serovar 1/2a, 5.8% to serovar 1/2b, 20.58% to serovar 1/2c, 5.8% to serovar 3b and 24.47% to serovar 4b.
D Art; P Andre
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Zentralblatt für Bakteriologie : international journal of medical microbiology     Volume:  275     ISSN:  0934-8840     ISO Abbreviation:  Zentralbl. Bakteriol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-12-24     Completed Date:  1991-12-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203851     Medline TA:  Zentralbl Bakteriol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  79-84     Citation Subset:  IM    
Institut d'Hygiène et d'Epidémiologie, Département Microbiologie, Bruxelles, Belgique.
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MeSH Terms
Colony Count, Microbial
Culture Media
Fish Products
Food Microbiology*
Listeria monocytogenes / growth & development,  isolation & purification*
Reg. No./Substance:
0/Culture Media

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