Document Detail


Comparison of textilinin-1 with aprotinin as serine protease inhibitors and as antifibrinolytic agents.
MedLine Citation:
PMID:  16707925     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Textilinin-1 (Q8008) was isolated from the venom of the Pseudonaja textilis and has a 47% sequence identity to the antihaemorrhagic therapeutic agent aprotinin. When equimolar concentrations of enzyme and aprotinin were pre-incubated, plasmin was inhibited 100%, plasma kallikrein 58%, and tissue kallikrein 99%. Under the same conditions, textilinin-1 inhibited plasmin 98%, plasma kallikrein 16% and tissue kallikrein 17%. Whole blood clot lysis was inhibited strongly by both aprotinin and textilinin-1, as shown by thrombelastography. At 2 microM inhibitor lysis initiated by t-PA was greater than 99% inhibited by aprotinin (LY60 = 0.4 +/- 0.1) whereas textilinin-1, inhibited lysis by 91% (LY60 = 8.9 +/- 0.7). The same trend was found with the lysis of euglobulin fractions. From these data textilinin-1 appears to be a more specific plasmin inhibitor than aprotinin but aprotinin inhibits clot lysis to a greater extent.
Authors:
Simone Flight; Lambro Johnson; Manuela Trabi; Patrick Gaffney; Martin Lavin; John de Jersey; Paul Masci
Related Documents :
24052575 - Factors associated with perinatal mortality in kassala, eastern sudan: a community-base...
16709575 - Dealing with innovation and costs in orthopedics: a conversation with dane miller. inte...
22422485 - Genetic variants modify susceptibility to leukemia in infants: a children's oncology gr...
24326505 - Jacob aall's illness and death.
8034395 - Nuclear fallout, low birthweight, and immune deficiency.
19757335 - Neonatal morbidity and mortality of late-preterm babies.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pathophysiology of haemostasis and thrombosis     Volume:  34     ISSN:  1424-8832     ISO Abbreviation:  Pathophysiol. Haemost. Thromb.     Publication Date:  2005  
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-07-31     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101142710     Medline TA:  Pathophysiol Haemost Thromb     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  188-93     Citation Subset:  IM    
Affiliation:
School of Medicine, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antifibrinolytic Agents / pharmacology*
Aprotinin / pharmacology*
Elapid Venoms / pharmacology*
Fibrinolysin / antagonists & inhibitors
Humans
Plasma Kallikrein / antagonists & inhibitors
Serine Proteinase Inhibitors / pharmacology*
Snake Venoms
Thrombelastography
Tissue Kallikreins / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Antifibrinolytic Agents; 0/Elapid Venoms; 0/Serine Proteinase Inhibitors; 0/Snake Venoms; 0/textilinin; 9087-70-1/Aprotinin; EC 3.4.21.34/Plasma Kallikrein; EC 3.4.21.35/Tissue Kallikreins; EC 3.4.21.7/Fibrinolysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Hemextin AB complex--a snake venom anticoagulant protein complex that inhibits factor VIIa activity.
Next Document:  Identification and localization of heparin-binding region of snake venom VEGF and its blocking of VE...