| Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany. | |
| | |
MedLine Citation:
|
PMID: 22389373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly of Alphaproteobacteria (26% of all phylotypes), Gammaproteobacteria (11%), candidate division TM6 (11%), Chlamydiales (9%), and Betaproteobacteria (9%). The bulk water community consisted primarily of Bacteroidetes (25%), Betaproteobacteria (20%), Actinobacteria (16%), and Alphaproteobacteria (11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity. |
| | |
Authors:
|
Karsten Henne; Leila Kahlisch; Ingrid Brettar; Manfred G Höfle |
Related Documents
:
|
12691913 - Use of fluorescent in situ hybridization to evidence the presence of helicobacter pylor... 12112633 - Quality of water types in ukraine evaluated by watertox bioassays. 15518513 - Enhancement of electrochemical activity by small-sizing the vinylferrocene-immobilized ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-03-02 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 78 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-04-26 Completed Date: 2012-08-15 Revised Date: 2013-02-19 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 3530-8 Citation Subset: IM |
Affiliation:
|
Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/FR796543; FR796544; FR796545; FR796546; FR796547; FR796548; FR796549; FR796550; FR796551; FR796552; FR796553; FR796554; FR796555; FR796556; FR796557; FR796558; FR796559; FR796560; FR796561; FR796562; FR796563; FR796564; FR796565; FR796566; FR796567; FR796568; FR796569; FR796570; FR796571; FR796572; FR796573; FR796574; FR796575; FR796576; FR796577; FR796578; FR796579; FR796580; FR796581; FR796582; FR796583; FR796584; FR796585; FR796586; FR796587; FR796588; FR796589; FR796590; FR796591; FR796592; FR796593; FR796594; FR796595; FR796596; FR796597; FR796598; FR796599; FR796600; FR796601; FR796602; FR796603; FR796604; FR796605; FR796606; FR796607; FR796608; FR796609; FR796610; FR796611; FR796612; FR796613; FR796614; FR796615; FR796616; FR796617; FR796618; FR796619; FR796620; FR796621; FR796622; FR796623; FR796624; FR796625; FR796626; FR796627; FR796628; FR796629; FR796630; FR796631; FR796632; FR796633; FR796634; FR796635; FR796636; FR796637; FR796638; FR796639; FR796640; FR796641; FR796642; FR796643; FR796644; FR796645; FR796646; FR796647; FR796648; FR796649; FR796650; FR796651; FR796652; FR796653; FR796654; FR796655; FR796656; FR796657; FR796658; FR796659; FR796660; FR796661; FR796662; FR796663; FR796664; FR796665; FR796666; FR796667; FR796668; FR796669; FR796670; FR796671; FR796672; FR796673; FR796674; FR796675; FR796676; FR796677; FR796678; FR796679; FR796680; FR796681; FR796682; FR796683; FR796684; FR796685; FR796686; FR796687; FR796688; FR796689; FR796690; FR796691; FR796692; FR796693; FR796694; FR796695; FR796696; FR796697; FR796698 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
classification*,
genetics Bacterial Physiological Phenomena* Biofilms / growth & development* Biota* Cluster Analysis DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics Drinking Water / microbiology* Germany Molecular Sequence Data Phylogeny Polymorphism, Single-Stranded Conformational RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Drinking Water; 0/RNA, Ribosomal, 16S |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Selection of a Bifidobacterium animalis subsp. lactis strain with a decreased ability to produce ace...
Next Document: Evidence of host-associated populations of Cryptosporidium parvum in Italy.