Document Detail


Analysis of structure and composition of bacterial core communities in mature drinking water biofilms and bulk water of a citywide network in Germany.
MedLine Citation:
PMID:  22389373     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly of Alphaproteobacteria (26% of all phylotypes), Gammaproteobacteria (11%), candidate division TM6 (11%), Chlamydiales (9%), and Betaproteobacteria (9%). The bulk water community consisted primarily of Bacteroidetes (25%), Betaproteobacteria (20%), Actinobacteria (16%), and Alphaproteobacteria (11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity.
Authors:
Karsten Henne; Leila Kahlisch; Ingrid Brettar; Manfred G Höfle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-03-02
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-26     Completed Date:  2012-08-15     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3530-8     Citation Subset:  IM    
Affiliation:
Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/FR796543;  FR796544;  FR796545;  FR796546;  FR796547;  FR796548;  FR796549;  FR796550;  FR796551;  FR796552;  FR796553;  FR796554;  FR796555;  FR796556;  FR796557;  FR796558;  FR796559;  FR796560;  FR796561;  FR796562;  FR796563;  FR796564;  FR796565;  FR796566;  FR796567;  FR796568;  FR796569;  FR796570;  FR796571;  FR796572;  FR796573;  FR796574;  FR796575;  FR796576;  FR796577;  FR796578;  FR796579;  FR796580;  FR796581;  FR796582;  FR796583;  FR796584;  FR796585;  FR796586;  FR796587;  FR796588;  FR796589;  FR796590;  FR796591;  FR796592;  FR796593;  FR796594;  FR796595;  FR796596;  FR796597;  FR796598;  FR796599;  FR796600;  FR796601;  FR796602;  FR796603;  FR796604;  FR796605;  FR796606;  FR796607;  FR796608;  FR796609;  FR796610;  FR796611;  FR796612;  FR796613;  FR796614;  FR796615;  FR796616;  FR796617;  FR796618;  FR796619;  FR796620;  FR796621;  FR796622;  FR796623;  FR796624;  FR796625;  FR796626;  FR796627;  FR796628;  FR796629;  FR796630;  FR796631;  FR796632;  FR796633;  FR796634;  FR796635;  FR796636;  FR796637;  FR796638;  FR796639;  FR796640;  FR796641;  FR796642;  FR796643;  FR796644;  FR796645;  FR796646;  FR796647;  FR796648;  FR796649;  FR796650;  FR796651;  FR796652;  FR796653;  FR796654;  FR796655;  FR796656;  FR796657;  FR796658;  FR796659;  FR796660;  FR796661;  FR796662;  FR796663;  FR796664;  FR796665;  FR796666;  FR796667;  FR796668;  FR796669;  FR796670;  FR796671;  FR796672;  FR796673;  FR796674;  FR796675;  FR796676;  FR796677;  FR796678;  FR796679;  FR796680;  FR796681;  FR796682;  FR796683;  FR796684;  FR796685;  FR796686;  FR796687;  FR796688;  FR796689;  FR796690;  FR796691;  FR796692;  FR796693;  FR796694;  FR796695;  FR796696;  FR796697;  FR796698
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification*,  genetics
Bacterial Physiological Phenomena*
Biofilms / growth & development*
Biota*
Cluster Analysis
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Drinking Water / microbiology*
Germany
Molecular Sequence Data
Phylogeny
Polymorphism, Single-Stranded Conformational
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Drinking Water; 0/RNA, Ribosomal, 16S
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