| Comparison of the structural stability and dynamic properties of recombinant anthrax protective antigen and its 2-fluorohistidine-labeled analogue. | |
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MedLine Citation:
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PMID: 22911632 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Protective antigen (PA) is the primary protein antigenic component of both the currently used anthrax vaccine and related recombinant vaccines under development. An analogue of recombinant PA (2-FHis rPA) has been recently shown to block the key steps of pore formation in the process of inducing cytotoxicity in cells, and thus can potentially be used as an antitoxin or a vaccine. This rPA analogue was produced by fermentation to incorporate the unnatural amino acid 2-fluorohistidine (2-FHis). In this study, the effects of 2-FHis labeling on rPA antigen's conformational stability and dynamic properties were investigated by various biophysical techniques. Temperature/pH stability profiles of rPA and 2-FHis rPA were analyzed by the empirical phase diagram (EPD) approach, and physical stability differences between them were identified. Results showed that rPA and 2-FHis rPA had similar stability at pH 7-8. With decreasing solution pH, however, 2-FHis rPA was found to be more stable. Dynamic sensitive measurements of the two proteins at pH 5 found that 2-FHis rPA was more dynamic and/or differentially hydrated under acidic pH conditions. The biophysical characterization and stability data provide information useful for the potential development of 2-FHis rPA as a more stable rPA vaccine candidate. © 2012 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci. |
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Authors:
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Lei Hu; Sangeeta B Joshi; Kiran K Andra; Santosh V Thakkar; David B Volkin; James G Bann; C Russell Middaugh |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-21 |
Journal Detail:
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Title: Journal of pharmaceutical sciences Volume: - ISSN: 1520-6017 ISO Abbreviation: J Pharm Sci Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985195R Medline TA: J Pharm Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Macromolecule and Vaccine Stabilization Center, Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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