Document Detail


Comparison of the structural stability and dynamic properties of recombinant anthrax protective antigen and its 2-fluorohistidine-labeled analogue.
MedLine Citation:
PMID:  22911632     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protective antigen (PA) is the primary protein antigenic component of both the currently used anthrax vaccine and related recombinant vaccines under development. An analogue of recombinant PA (2-FHis rPA) has been recently shown to block the key steps of pore formation in the process of inducing cytotoxicity in cells, and thus can potentially be used as an antitoxin or a vaccine. This rPA analogue was produced by fermentation to incorporate the unnatural amino acid 2-fluorohistidine (2-FHis). In this study, the effects of 2-FHis labeling on rPA antigen's conformational stability and dynamic properties were investigated by various biophysical techniques. Temperature/pH stability profiles of rPA and 2-FHis rPA were analyzed by the empirical phase diagram (EPD) approach, and physical stability differences between them were identified. Results showed that rPA and 2-FHis rPA had similar stability at pH 7-8. With decreasing solution pH, however, 2-FHis rPA was found to be more stable. Dynamic sensitive measurements of the two proteins at pH 5 found that 2-FHis rPA was more dynamic and/or differentially hydrated under acidic pH conditions. The biophysical characterization and stability data provide information useful for the potential development of 2-FHis rPA as a more stable rPA vaccine candidate.
Authors:
Lei Hu; Sangeeta B Joshi; Kiran K Andra; Santosh V Thakkar; David B Volkin; James G Bann; C Russell Middaugh
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-21
Journal Detail:
Title:  Journal of pharmaceutical sciences     Volume:  101     ISSN:  1520-6017     ISO Abbreviation:  J Pharm Sci     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-18     Completed Date:  2013-02-07     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  2985195R     Medline TA:  J Pharm Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4118-28     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Macromolecule and Vaccine Stabilization Center, Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66047, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / chemistry*
Bacterial Toxins / chemistry*
Circular Dichroism
Histidine / analogs & derivatives*,  chemistry
Hydrogen-Ion Concentration
Recombinant Proteins / chemistry
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Temperature
Grant Support
ID/Acronym/Agency:
5P20 RR17708/RR/NCRR NIH HHS; P20 RR017708/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Toxins; 0/Recombinant Proteins; 0/anthrax toxin; 57212-36-9/2-fluorohistidine; 71-00-1/Histidine
Comments/Corrections

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