Document Detail

Comparison of sputum induction using inhaled methacholine or hypertonic saline.
MedLine Citation:
PMID:  15691239     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Hypertonic saline is recommended for the induction of sputum to assess airway inflammation. Methacholine is most commonly used to assess airway responsiveness. The aim of this study was to compare sputum induction by methacholine and hypertonic saline challenges, and the subsequent inflammatory cell differential counts. METHODOLOGY: Subjects (n = 56) underwent inhaled hypertonic saline (4.5% for < or = 15 min without bronchodilator pretreatment) and methacholine (maximum cumulative dose 4 micromol) challenges 1 week apart, in random order. Sputum was processed, and on cytospins stained with May-Grunwald-Giemsa, a total of 400 inflammatory plus squamous cells were counted to obtain a differential cell count. RESULTS: A satisfactory sputum sample was obtained from 29 subjects following hypertonic saline and also from 10 subjects following methacholine. For these 10 subjects, the mean percent macrophages, neutrophils, eosinophils, and lymphocytes were similar after methacholine (64%, 26%, 7% and 1%) or hypertonic saline (67%, 26%, 6% and 1%). The results were similar for squamous cells (42% and 45%) and when asthmatics and nonasthmatics were compared separately. CONCLUSIONS: Inhaled methacholine has a much lower yield of sputum induction compared with hypertonic saline. However, the differential sputum cell count is similar following both challenge agents.
Alan L James; Peta S Maxwell; John G Elliot
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Publication Detail:
Type:  Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Respirology (Carlton, Vic.)     Volume:  10     ISSN:  1323-7799     ISO Abbreviation:  Respirology     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-02-04     Completed Date:  2005-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9616368     Medline TA:  Respirology     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  57-62     Citation Subset:  IM    
West Australian Sleep Disorders Research Institute, Sir Charles Gairdner Hospital, Nedlands, Western Australia, Australia.
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MeSH Terms
Asthma / pathology,  physiopathology
Bronchial Provocation Tests / methods*
Bronchoconstriction / drug effects
Bronchoconstrictor Agents / pharmacology*
Cell Count
Coloring Agents / diagnostic use
Eosinophils / cytology
Epithelial Cells / cytology
Leukocyte Count
Lymphocyte Count
Lymphocytes / cytology
Macrophages / cytology
Methacholine Chloride / pharmacology*
Middle Aged
Neutrophils / cytology
Saline Solution, Hypertonic / pharmacology*
Sputum / cytology,  drug effects*
Reg. No./Substance:
0/Bronchoconstrictor Agents; 0/Coloring Agents; 0/Saline Solution, Hypertonic; 62-51-1/Methacholine Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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