| Comparison of sputum induction using inhaled methacholine or hypertonic saline. | |
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MedLine Citation:
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PMID: 15691239 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Hypertonic saline is recommended for the induction of sputum to assess airway inflammation. Methacholine is most commonly used to assess airway responsiveness. The aim of this study was to compare sputum induction by methacholine and hypertonic saline challenges, and the subsequent inflammatory cell differential counts. METHODOLOGY: Subjects (n = 56) underwent inhaled hypertonic saline (4.5% for < or = 15 min without bronchodilator pretreatment) and methacholine (maximum cumulative dose 4 micromol) challenges 1 week apart, in random order. Sputum was processed, and on cytospins stained with May-Grunwald-Giemsa, a total of 400 inflammatory plus squamous cells were counted to obtain a differential cell count. RESULTS: A satisfactory sputum sample was obtained from 29 subjects following hypertonic saline and also from 10 subjects following methacholine. For these 10 subjects, the mean percent macrophages, neutrophils, eosinophils, and lymphocytes were similar after methacholine (64%, 26%, 7% and 1%) or hypertonic saline (67%, 26%, 6% and 1%). The results were similar for squamous cells (42% and 45%) and when asthmatics and nonasthmatics were compared separately. CONCLUSIONS: Inhaled methacholine has a much lower yield of sputum induction compared with hypertonic saline. However, the differential sputum cell count is similar following both challenge agents. |
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Authors:
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Alan L James; Peta S Maxwell; John G Elliot |
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Publication Detail:
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Type: Clinical Trial; Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Respirology (Carlton, Vic.) Volume: 10 ISSN: 1323-7799 ISO Abbreviation: Respirology Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-02-04 Completed Date: 2005-06-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9616368 Medline TA: Respirology Country: Australia |
Other Details:
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Languages: eng Pagination: 57-62 Citation Subset: IM |
Affiliation:
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West Australian Sleep Disorders Research Institute, Sir Charles Gairdner Hospital, Nedlands, Western Australia, Australia. ajames@it.net.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Asthma / pathology, physiopathology Bronchial Provocation Tests / methods* Bronchoconstriction / drug effects Bronchoconstrictor Agents / pharmacology* Cell Count Coloring Agents / diagnostic use Eosinophils / cytology Epithelial Cells / cytology Female Humans Leukocyte Count Lymphocyte Count Lymphocytes / cytology Macrophages / cytology Male Methacholine Chloride / pharmacology* Middle Aged Neutrophils / cytology Saline Solution, Hypertonic / pharmacology* Sputum / cytology, drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Bronchoconstrictor Agents; 0/Coloring Agents; 0/Saline Solution, Hypertonic; 62-51-1/Methacholine Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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