Document Detail


Comparison of some of the physicochemical characteristics of type 2 diabetic and normal human bones: a sample study.
MedLine Citation:
PMID:  20801059     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AIM: The aim of this study was to compare some of the physicochemical characteristics of type 2 diabetic bones (DBs) and normal bones (NBs).
MATERIALS AND METHODS: The organic and inorganic parts of human NBs and DBs were separated using conventional methods, and their physicochemical characteristics were compared using infrared (IR) spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, thermogravimetric analysis, X-ray diffraction (XRD), and scanning electron microscopy (SEM).
RESULTS: The IR spectrum of the collagen part of DBs has showed the presence of carbonyl groups, indicating cross-linking in the α-chain. This was also confirmed by the increased thermal stability (22°C) in DB collagen. The XRD data of the inorganic part of DBs have revealed decreased crystallinity. SEM images of the inorganic part of DBs have shown a porous (weak) nature compared to those of NBs, which exhibited a compact (healthy) nature.
CONCLUSION: The cross-linking in DB collagen molecules and the decreased and defective crystallinity in the inorganic portion of DBs might be the reasons for the increased risk of fracture among diabetic patients.
Authors:
Santhanam Sekar; Annamalai Chandrasekaran; Urmila Rao; Thotapalli Parvathaleswara Sastry
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Publication Detail:
Type:  Journal Article     Date:  2010-08-30
Journal Detail:
Title:  Journal of diabetes and its complications     Volume:  25     ISSN:  1873-460X     ISO Abbreviation:  J. Diabetes Complicat.     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-04-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9204583     Medline TA:  J Diabetes Complications     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Bio-products Laboratory, Central Leather Research Institute, Chennai 600 020, India.
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