| Comparison of somatostatin-28 and somatostatin-14 clearance by the perfused rat liver. | |
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MedLine Citation:
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PMID: 2859104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hepatic clearances of somatostatin (SS)-28 and SS-14 by the perfused rat liver were compared, using a recirculating, plasma-free, erythrocyte-containing perfusion system. The disappearance rate constant, half time, clearance, and hepatic extraction ratio when 1.2 nM SS-28 was added to the perfusate were 0.0221 +/- 0.0051 min-1, 36.6 +/- 7.6 min, 0.34 +/- 0.08 mL/min, and 17.2 +/- 3.9%, respectively. The corresponding values obtained when SS-14 was added to the perfusate were 0.0405 +/- 0.0022 min-1, 17.3 +/- 1.0 min, 0.71 +/- 0.05 mL/min, and 35.4 +/- 2.6%, respectively. The differences between the SS-28 and SS-14 indices were all statistically significant. In addition, the perfusates with SS-28 added were eluted on Sephadex G-25 fine columns and somatostatinlike immunoreactivity (SLI) was determined. No SS-14 was found in perfusate containing SS-28 at both 5 and 30 min after the beginning of the perfusion. To investigate whether or not the liver plays an important role in the clearance of SS-28 or the conversion of SS-14 in vivo, the plasma disappearance of 2 micrograms SS-28 was compared in the whole rat and the functionally hepatectomized model. The half time of plasma SS-28 was 1.43 +/- 0.12 min in the whole rat, significantly shorter than the 2.20 +/- 0.14 min in the hepatectomized model. Gel filtration of plasma extract samples at 0.5 min after the SS-28 injection showed two major peaks of SLI: a first peak corresponding to SS-28 and a second peak coeluted in the position of SS-14 in both the whole rat and the hepatectomized model. At 4 min after the SS-28 injection, the first peak disappeared and only a small second peak was observed.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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M Seno; Y Seino; Y Takemura; S Nishi; H Ishida; N Kitano; H Imura; T Taminato; S Matsukura |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 63 ISSN: 0008-4212 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-06-03 Completed Date: 1985-06-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: CANADA |
Other Details:
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Languages: eng Pagination: 62-7 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gel Liver / metabolism* Male Perfusion Rats Rats, Inbred Strains Somatostatin / metabolism* Somatostatin-28 |
| Chemical | |
Reg. No./Substance:
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51110-01-1/Somatostatin; 75037-27-3/Somatostatin-28 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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