Document Detail


Comparison of single nucleotide polymorphisms in the human interleukin-10 gene promoter between rheumatoid arthritis patients and normal subjects in Malaysia.
MedLine Citation:
PMID:  17929139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, three single nucleotide polymorphisms (SNPs) located within the promoter of the human interleukin (IL)-10 gene [rs1800896 (position: -1087G>A), rs1800871 (position: -824C>T) and rs1800872 (position: -597C>A)] were investigated in 84 rheumatoid arthritis (RA) patients and 95 age- and sex-matched healthy subjects using polymerase chain reaction-restriction fragment length polymorphism method. Production of IL-10 by peripheral blood lymphocytes from the RA patients and healthy subjects cultured in the presence of Concanavalin A (Con A) was determined by using enzyme-linked immunosorbent assay. The results show that the distribution of the IL-10 genotypes did not differ significantly between RA patients and healthy subjects (P>0.05). However, a significant difference was observed in allele frequencies of -824CT, -824TT, -597CA, and -597AA between the RA patients and healthy volunteers (P=0.04). The -1087A/-824T/-597A (ATA) haplotype, which comprises all mutant alleles, was associated with lower IL-10 production when compared with the other haplotypes. In contrast, the RA patients who did not display the ATA haplotype produced significantly higher levels of IL-10 when compared with those carrying either one (P=0.012) or two (P=0.005) ATA haplotypes. Our findings suggest that there is an association between SNPs in the promoter of the human IL-10 gene and susceptibility to RA.
Authors:
Chee Seng Hee; Suk Chyn Gun; Rakesh Naidu; Esha Gupta; Sushela Devi Somnath; Ammu Kutty Radhakrishnan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-19
Journal Detail:
Title:  Modern rheumatology / the Japan Rheumatism Association     Volume:  17     ISSN:  1439-7595     ISO Abbreviation:  Mod Rheumatol     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-11     Completed Date:  2008-01-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100959226     Medline TA:  Mod Rheumatol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  429-35     Citation Subset:  IM    
Affiliation:
International Medical University, No. 126, Jalan 19/155B, Bukit Jalil, 57000, Kuala Lumpur, and Department of Medicine, Hospital Tuanku Ja'afar, Seremban, Malaysia. cs_hee@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Alleles
Arthritis, Rheumatoid / diagnosis*,  genetics*
Case-Control Studies
Concanavalin A / pharmacology
Enzyme-Linked Immunosorbent Assay
Female
Gene Frequency
Genetic Predisposition to Disease*
Haplotypes
Humans
Interleukin-10 / genetics*
Malaysia
Male
Polymorphism, Single Nucleotide*
Promoter Regions, Genetic*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
11028-71-0/Concanavalin A; 130068-27-8/Interleukin-10
Comments/Corrections
Erratum In:
Mod Rheumatol. 2007;17(6):534

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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