Document Detail


Comparison of silicon-coated nylon suture to plain nylon suture in the rat middle cerebral artery occlusion model.
MedLine Citation:
PMID:  16569436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A variety of intraluminal sutures have been used in the middle cerebral artery occlusion model (MCAO) of focal ischemia. In the present study we tested commercially available silicon-coated nylon suture in the MCAO model and compared the results to traditional monofilament nylon suture occlusion. Twelve Sprague-Dawley male rats were randomly divided two groups, MCAO with 4-0 nylon suture (Group N, n=6) and MCAO with silicone-coated 4-0 nylon suture (Group S, n=6). Rats were sacrificed 24 h after reperfusion. Assessment included mortality rates, neurological evaluation, and infarct volume. One rat died in each group from subarachanoid hemorrhage. Neurological evaluation demonstrated that Group S tended to have worse neurological outcomes than Group N, although this difference was not statistically significant. On TTC stain Group S had significantly larger infarct volumes than Group N. We conclude that the commercially available silicone-coated occlusion suture provides better occlusion of the middle cerebral artery than the traditional uncoated nylon suture. Classification: Disease-related neuroscience (Section 6).
Authors:
Norihito Shimamura; Gerald Matchett; Tamiji Tsubokawa; Hiroki Ohkuma; John Zhang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2006-03-29
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  156     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-24     Completed Date:  2006-11-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  161-5     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal / physiology
Brain / pathology
Brain Ischemia / pathology,  psychology
Infarction, Middle Cerebral Artery / pathology*,  psychology
Ligation
Male
Middle Cerebral Artery / physiology*
Nylons*
Rats
Rats, Sprague-Dawley
Silicon*
Sutures*
Grant Support
ID/Acronym/Agency:
HD43120/HD/NICHD NIH HHS; NS43338/NS/NINDS NIH HHS; NS45694/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nylons; 7440-21-3/Silicon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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