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Comparison of sensitization between beta-lactoglobulin and its hydrolysates.
MedLine Citation:
PMID:  22523905     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
BACKGROUND: Bovine beta-lactoglobulin is one of the first foreign antigens encountered by some newborn children, and it has been described as the main allergenic protein in cow's milk even when present at low concentration.
OBJECTIVE: Enzymatic hydrolysis has been identified to be a very effective way to reduce the sensitization of beta-lg compared to other treatments. The aim of this study was to explore whether enzymatic hydrolysis could reduce the allergenicity of native bovine beta-lg.
METHODS: beta-lg was hydrolyzed by trypsin. Twenty four BALB/c mice were divided into three groups and orally sensitized by native bovine beta-lg and its hydrolysates five times at weekly intervals.
RESULTS: During the sensitization period, many serious systemic anaphylactic symptoms were observed in mice sensitized by native beta-lg compared to hydrolysates of beta-lg. Mice sensitized by hydrolysates of beta-lg showed a significantly lower spleen lymphocyte proliferation level than intact beta-lg. The beta-lg-specific IgE antibody levels in serum and intestinal fluid samples induced by native beta-lg were significantly elevated. Plasma histamine levels were also evaluated and showed the same trend as IgE. Moreover, the hydrolysates of beta-lg significantly up-regulated IFN-gamma and IL-10 production and down-regulated IL-4 and IL-5 secretions by murine splenocytes.
CONCLUSION: These results suggested that enzymatic hydrolysis could partly reduce the allergenicity of beta-lg.
Authors:
Cuicui Duan; Guicheng Huo; Lijie Yang; Daxi Ren; Junliang Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand     Volume:  30     ISSN:  0125-877X     ISO Abbreviation:  Asian Pac. J. Allergy Immunol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8402034     Medline TA:  Asian Pac J Allergy Immunol     Country:  Thailand    
Other Details:
Languages:  eng     Pagination:  32-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.
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