Document Detail

Comparison of the sensitivity of human embryo kidney cells, HeLa cells, and WI38 cells for the primary isolation of viruses from the eye.
MedLine Citation:
PMID:  168234     Owner:  NLM     Status:  MEDLINE    
A comparison has been made of the efficiency of human embryo kidney (HEK) cills, HeLa cells, and WI38 cells for the isolation of viruses from the eyes of patients suffering from acute conjunctivitis or keratoconjunctivitis. From a total of 99 specimens 21 adenoviruses (serotypes 3, 4, 7, 8, and 13) were isolated in HEK cells, eight (serotypes 3 and 8) in HeLa cells, and four (serotype 3) in WI38 cells. Of the ten herpes simplex viruses isolated nine were recovered in HEK cells, seven in WI38 cells, and none in HeLa cells. The combination of HeLa cells and WI38 cells is not considered an adequate alternative to the difficult-to-obtain HEK cells for the isolation of viruses from the eye.
D A McSwiggan; S Darougar; A F Rahman; J A Gibson
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  28     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-11-06     Completed Date:  1975-11-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  410-3     Citation Subset:  AIM; IM    
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MeSH Terms
Adenoviridae / isolation & purification*
Cell Line*
Conjunctivitis / microbiology*
Cytopathogenic Effect, Viral*
Hela Cells*
Keratoconjunctivitis / microbiology*
Kidney / embryology
Lung / embryology
Simplexvirus / isolation & purification*
Virus Cultivation / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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