Document Detail

Comparison of the response of primary human blood monocytes and the U937 human monocytic cell line to two different sizes of alumina ceramic particles.
MedLine Citation:
PMID:  15183442     Owner:  NLM     Status:  MEDLINE    
It is well recognized that wear particles derived from orthopaedic implants have the potential to induce inflammation, which may eventually lead to aseptic loosening of the artificial joint. We hypothesized that alumina ceramic particles of different sizes cause a differential cytokine response by human monocytes. To test this hypothesis a human monocytic cell line (U937) and primary human blood monocytes obtained from healthy volunteers were exposed to ceramic particles within the range known to be generated in vivo. Cellular responses were measured by quantifying the relative gene expression of 12 different cytokines using TAQman Real-Time Polymerase Chain Reaction (RT-PCR). Our results demonstrate that at a particle to cell ratio of 100:1, 0.5 microm ceramic particles consistently provoked higher amounts of Interleukin-1alpha (IL-1alpha), IL-1beta, IL-8, IL-10 and Tumor necrosis factor-alpha (TNF-alpha) steady state mRNA by U937 cells. As expected, the variability of cytokine expression in primary blood monocytes was much higher compared to the cell line however, a similar trend was observed. These results show a differential response to ceramic particle size, which may imply that 0.5 microm particles are less biocompatible. New ceramic implants can be designed to generate a known particle size range in vivo. Implant materials of this type may induce relatively lower levels of production of inflammatory cytokines resulting in a reduced incidence of failure due to aseptic loosening.
Efrat Yagil-Kelmer; Peter Kazmier; Mohamed N Rahaman; B Sonny Bal; Ronald K Tessman; D Mark Estes
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of orthopaedic research : official publication of the Orthopaedic Research Society     Volume:  22     ISSN:  0736-0266     ISO Abbreviation:  J. Orthop. Res.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-08     Completed Date:  2004-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8404726     Medline TA:  J Orthop Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  832-8     Citation Subset:  IM    
Department of Veterinary Pathobiology, University of Missouri, 1600 East Rollins Road, Columbia, MO 65211, USA.
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MeSH Terms
Aluminum / metabolism,  pharmacology*
Biocompatible Materials / metabolism,  pharmacology*
Cell Survival / drug effects
Ceramics / metabolism,  pharmacology*
Cytokines / genetics,  metabolism
Dose-Response Relationship, Drug
Monocytes / drug effects*,  metabolism
Particle Size
Phagocytosis / drug effects
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods
U937 Cells / drug effects*,  metabolism
Reg. No./Substance:
0/Biocompatible Materials; 0/Ceramics; 0/Cytokines; 0/RNA, Messenger; 7429-90-5/Aluminum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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