Document Detail

Comparison of a pump-around, a diffusion-driven, and a shear-driven system for the hybridization of mouse lung and testis total RNA on microarrays.
MedLine Citation:
PMID:  16196108     Owner:  NLM     Status:  MEDLINE    
In the present study, we demonstrate the benefits of a shear-driven rotating microchamber system for the enhancement of microarray hybridizations, by comparing the system with two commonly used hybridization techniques: purely diffusion-driven hybridization under coverslip and hybridization using a fully automated hybridization station, in which the sample is pumped in an oscillating manner. Starting from the same amount of DNA for the three different methods, a series of hybridization experiments using mouse lung and testis DNA is presented to demonstrate these benefits. The gain observed using the rotating microchamber is large: both in terms of analysis speed (up to tenfold increase) and in final spot intensity (up to sixfold increase). The gain is due to the combined effect of the hybridization chamber miniaturization (leading to a sample concentration increase if comparing iso-mass conditions) and the transport enhancement originating from the rotational shear-driven flow induced by the rotation of the chamber bottom wall.
Johan Vanderhoeven; Kris Pappaert; Binita Dutta; Paul Van Hummelen; Gert Desmet
Related Documents :
23483558 - Integrated chip electrophoresis and magnetic particle isolation used for detection of h...
3133178 - A human-derived probe, p82h, hybridizes to the centromeres of gorilla, chimpanzee, and ...
2654808 - Detection of proto-oncogenes in the genome of the amphibian xenopus laevis.
17464398 - Dna meets synthetic polymers--highly versatile hybrid materials.
22929698 - Zeptomole detection of dna nanoparticles by single-molecule fluorescence with magnetic ...
185408 - Methylation of high-molecular-weight subunit rna of feline leukemia virus.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  26     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-11     Completed Date:  2005-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3773-9     Citation Subset:  IM    
Department of Chemical Engineering, Vrije Universiteit Brussel, Brussels, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Lung / chemistry*
Miniaturization / instrumentation
Oligonucleotide Array Sequence Analysis / instrumentation,  methods*
RNA / analysis*
Testis / chemistry*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Parallel mixing of photolithographically defined nanoliter volumes using elastomeric microvalve arra...
Next Document:  Standardization of uveitis nomenclature for reporting clinical data. Results of the First Internatio...