| Comparison of a pump-around, a diffusion-driven, and a shear-driven system for the hybridization of mouse lung and testis total RNA on microarrays. | |
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MedLine Citation:
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PMID: 16196108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present study, we demonstrate the benefits of a shear-driven rotating microchamber system for the enhancement of microarray hybridizations, by comparing the system with two commonly used hybridization techniques: purely diffusion-driven hybridization under coverslip and hybridization using a fully automated hybridization station, in which the sample is pumped in an oscillating manner. Starting from the same amount of DNA for the three different methods, a series of hybridization experiments using mouse lung and testis DNA is presented to demonstrate these benefits. The gain observed using the rotating microchamber is large: both in terms of analysis speed (up to tenfold increase) and in final spot intensity (up to sixfold increase). The gain is due to the combined effect of the hybridization chamber miniaturization (leading to a sample concentration increase if comparing iso-mass conditions) and the transport enhancement originating from the rotational shear-driven flow induced by the rotation of the chamber bottom wall. |
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Authors:
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Johan Vanderhoeven; Kris Pappaert; Binita Dutta; Paul Van Hummelen; Gert Desmet |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Electrophoresis Volume: 26 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-11 Completed Date: 2005-11-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: Germany |
Other Details:
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Languages: eng Pagination: 3773-9 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Vrije Universiteit Brussel, Brussels, Belgium. johan.vanderhoeven@vub.ac.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Lung / chemistry* Male Mice Miniaturization / instrumentation Oligonucleotide Array Sequence Analysis / instrumentation, methods* RNA / analysis* Testis / chemistry* |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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