Document Detail


Comparison of a pump-around, a diffusion-driven, and a shear-driven system for the hybridization of mouse lung and testis total RNA on microarrays.
MedLine Citation:
PMID:  16196108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the present study, we demonstrate the benefits of a shear-driven rotating microchamber system for the enhancement of microarray hybridizations, by comparing the system with two commonly used hybridization techniques: purely diffusion-driven hybridization under coverslip and hybridization using a fully automated hybridization station, in which the sample is pumped in an oscillating manner. Starting from the same amount of DNA for the three different methods, a series of hybridization experiments using mouse lung and testis DNA is presented to demonstrate these benefits. The gain observed using the rotating microchamber is large: both in terms of analysis speed (up to tenfold increase) and in final spot intensity (up to sixfold increase). The gain is due to the combined effect of the hybridization chamber miniaturization (leading to a sample concentration increase if comparing iso-mass conditions) and the transport enhancement originating from the rotational shear-driven flow induced by the rotation of the chamber bottom wall.
Authors:
Johan Vanderhoeven; Kris Pappaert; Binita Dutta; Paul Van Hummelen; Gert Desmet
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  26     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-11     Completed Date:  2005-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3773-9     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, Vrije Universiteit Brussel, Brussels, Belgium. johan.vanderhoeven@vub.ac.be
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MeSH Terms
Descriptor/Qualifier:
Animals
Lung / chemistry*
Male
Mice
Miniaturization / instrumentation
Oligonucleotide Array Sequence Analysis / instrumentation,  methods*
RNA / analysis*
Testis / chemistry*
Chemical
Reg. No./Substance:
63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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