Document Detail

Comparison of proteoglycans extracted by saline and guanidinium chloride from cultured chick retinas.
MedLine Citation:
PMID:  7334378     Owner:  NLM     Status:  MEDLINE    
To compare the loosely associated sulfated proteoglycans with those tightly bound to membranes, retinas from 14-day chick embryos were subjected to progressively disruptive techniques. The most easily removed proteoglycans were isolated from the medium in which the tissue was labeled with [35S]sulfate. On the average, 25% of the glycosaminoglycans were in the labeling medium, 39% were in proteoglycans extracted from the tissue in the balanced salt solution, 32% were in a 4 M-guanidinium chloride (GuCl) fraction, and 4% remained unextracted. These glycosaminoglycans contained, respectively, 28, 28, 40, and 4% of the incorporated [35S]sulfate. On the basis of electrophoretic mobility and TLC of chondroitinase digests, the ratio of 35S in chondroitin sulfate to that in heparan sulfate was 4-7 times higher in the medium and balanced salt extracts than in the GuCl extracts. In both extracts there was more 35S in chondroitin-6-sulfate than in chondroitin-4-sulfate. Dialysis of the extracts against 0.5 M-NaCl resulted in the precipitation of about 12% of the glycosaminoglycans in the saline extracts and about 40% in GuCl extract. These subfractions, which were relatively enriched in heparan sulfate, were largely soluble in dithiothreitol in 8 M-urea (DTT). Similarities between the proteoglycans in the medium and those extracted by balanced salt solutions suggest that the saline-extracted proteoglycans were for the most part loosely associated with cell surfaces of extracellular matrices, whereas the GuCl-extracted proteoglycans probably were bound to membranes.
J E Morris; Y P Ting
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  37     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-05-27     Completed Date:  1982-05-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1594-602     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Chick Embryo
Electrophoresis, Cellulose Acetate
Glycosaminoglycans / isolation & purification
Indicators and Reagents
Proteoglycans / isolation & purification*
Retina / analysis*
Sodium Chloride
Grant Support
Reg. No./Substance:
0/Glycosaminoglycans; 0/Guanidines; 0/Indicators and Reagents; 0/Proteoglycans; 113-00-8/Guanidine; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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