| Comparison of prostate cancer cell lines for androgen receptor-mediated reporter gene assays. | |
| | |
MedLine Citation:
|
PMID: 16621434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In order to select a better prostate cancer cell model for androgen receptor (AR)-mediated reporter gene assays, we assessed the androgen response characteristics of three cell lines, LNCaP, PC3/AR(+) and 22Rv1, in this study. Both the mRNA and the proteins of AR and glucocorticoid receptor (GR) were expressed in all three cell lines. Among the three cell lines, only in LNCaP cells, DHT concentration-dependently stimulated proliferation. DHT induced the luciferase activity in three cell lines which were transiently transfected with pMMTV-Luc, in a concentration-dependent manner. The maximum induction was 24.0-fold and 13.4-fold in 22Rv1 and in the LNCaP respectively. PC3/AR(+) were more sensitive to respond to DHT at a minimal concentration of 10(-12)M by 14.0-fold induction. The transcriptional activity induced with 10(-8)M DHT was inhibited about 50-75% in the PC3/AR(+) and 22Rv1, and 98% in the LNCaP, by vinclozolin. Dexamethasone concentration-dependently induced the luciferase activity in PC3 and 22Rv1, but not in the LNCaP. However, the response to dexamethasone in 22Rv1 was very weak compared to DHT. The (anti)androgencity of seven pyrethroids was assessed via an AR-mediated luciferase reporter assay. None of them showed the androgenic action in all three cell lines. Permethrin inhibited the DHT induced luciferase activity about 22%, 35.8% and 75.5% in 22Rv1, PC3/AR(+) and LNCaP, respectively. Based on results from in this study and cell line character, 22Rv1 cells seemed to be an appropriate model for the screening of androgenic endocrine disruptors, although it needs further studies with other steroid receptor and thyroid receptor. |
| | |
Authors:
|
Hyun-Jung Kim; Young In Park; Mi-Sook Dong |
Related Documents
:
|
7579484 - Influence of n-3 fatty acids on the growth of human breast cancer cells in vitro: relat... 9027364 - Androgen-dependent cell cycle arrest and apoptotic death in pc-3 prostatic cell culture... 14532964 - Reduced expression of the regulatory a subunit of serine/threonine protein phosphatase ... 7003594 - Immunocytochemical localization of the sex steroid-binding protein of plasma in tissues... 14756824 - Overexpression of torsina in pc12 cells protects against toxicity. 23277534 - Centrosome positioning in vertebrate development. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-08 |
Journal Detail:
|
Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 20 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2006 Oct |
Date Detail:
|
Created Date: 2006-07-24 Completed Date: 2007-01-26 Revised Date: 2009-04-10 |
Medline Journal Info:
|
Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
|
Languages: eng Pagination: 1159-67 Citation Subset: IM |
Affiliation:
|
School of Life Sciences and Biotechnology, Korea University, 1, 5-Ka, Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Androgen Antagonists
/
pharmacology Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Dexamethasone / pharmacology Dihydrotestosterone / analogs & derivatives, pharmacology Dose-Response Relationship, Drug Fungicides, Industrial / pharmacology Gene Expression / drug effects Humans Immunoblotting Insecticides / pharmacology Luciferases / genetics, metabolism* Male Oxazoles / pharmacology Prostatic Neoplasms / genetics, metabolism, pathology Pyrethrins / pharmacology RNA, Messenger / genetics, metabolism Receptors, Androgen / genetics, metabolism* Receptors, Glucocorticoid / genetics, metabolism Recombinant Fusion Proteins / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Transcriptional Activation / drug effects |
| Chemical | |
Reg. No./Substance:
|
0/Androgen Antagonists; 0/Fungicides, Industrial; 0/Insecticides; 0/Oxazoles; 0/Pyrethrins; 0/RNA, Messenger; 0/Receptors, Androgen; 0/Receptors, Glucocorticoid; 0/Recombinant Fusion Proteins; 0/glucocorticoid receptor alpha; 50-02-2/Dexamethasone; 50471-44-8/vinclozolin; 521-18-6/Dihydrotestosterone; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Neoadjuvant treatment of gastric cancer with peritoneal dissemination.
Next Document: Effects of moniliformin in presence of cyclohexadepsipeptides on isolated mammalian tissue and cells...