| Comparison of the properties and concentrations of the isoforms of retinol-binding protein in animals and human beings. | |
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MedLine Citation:
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PMID: 8214886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used size-exclusion high-performance liquid chromatography (HPLC) to investigate the properties of the 2 isoforms of Vitamin A-containing (holo) retinol-binding protein (RBP) in animals: the form that is bound to transthyretin (holo-TTR-RBP), and the form that does not bind to TTR (holo-free RBP). We also used radial immunodiffusion to measure immunologically active RBP (apo + holo RBP). We compared the isoforms of RBP in animals with those of human beings to determine which animal is the best model of human RBP. Size-exclusion HPLC detected holo-free and holo-TTR-RBP in every animal species studied. Apparent concentration of holo-TTR-RBP varied among species: that of rabbits and dogs >> that of apes, sheep, goats, monkeys, rhinoceroses, felids, rats, human beings, and deer > that of pigs, zebra, and bison > that of penguins. Dogs have unusual RBP chromatograms; they have high concentration of RBP, but also appear to transport much of their vitamin A on protein other than RBP. Human RBP antibody preparations could detect apo + holo RBP immunologic activity only in apes, monkeys, and felids. Apes and monkeys appeared to have complete cross-reactivity to human RBP antibodies. Felids may have substantial, but partial, cross-reactivity. Apes and monkeys appear to be the most relevant animal models for study of human RBP transport. However, there is a need for less-expensive models. Further research is needed, but in the interim, rats or sheep may be satisfactory for some purposes. |
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Authors:
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B J Burri; T R Neidlinger; H Zwick |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: American journal of veterinary research Volume: 54 ISSN: 0002-9645 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 1993 Aug |
Date Detail:
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Created Date: 1993-11-04 Completed Date: 1993-11-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1213-20 Citation Subset: IM |
Affiliation:
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Micronutrients Unit, Western Human Nutrition Research Center, USDA, ARS, Presidio of San Francisco, CA 94129. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Artiodactyla Birds Carnivora Chromatography, High Pressure Liquid / veterinary* Humans Macropodidae Perissodactyla Primates Rabbits Rats Retinol-Binding Proteins / analysis, chemistry* Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Retinol-Binding Proteins |
| Comments/Corrections | |
Erratum In:
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Am J Vet Res 1993 Sep;54(9):1559 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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