| Comparison of pretreatment strategies for enzymatic saccharification and fermentation of barley straw to ethanol. | |
| | |
MedLine Citation:
|
PMID: 19874923 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Barley straw used in this study contained 34.3% cellulose, 23.0% hemicellulose and 13.3% lignin (moisture, 6.5%). Several pretreatments (dilute acid, lime and alkaline peroxide) and enzymatic saccharification procedures were evaluated for the conversion of barley straw to monomeric sugars. The maximum release of sugars (glucose, 384 mg; xylose, 187 mg; arabinose, 32 mg; total sugars, 604 mg/g; 94% of maximum theoretical sugar yield) from barley straw (10%, w/v) was obtained by alkaline peroxide (2.5% H(2)O(2), pH 11.5) pretreatment (35 degrees C, 24 hours) and enzymatic saccharification (45 degrees C, pH 5.0, 120 hours) after diluting 2 times before adding a cocktail of three commercial enzyme preparations (cellulase, beta-glucosidase and hemicellulase) each at the dose level of 0.15 ml/g of straw. Dilute acid and lime pretreatments followed by enzymatic saccharification generated 566 mg (88% yield) and 582 mg (91% yield) total sugars/g of barley straw, respectively. The yield of ethanol from the dilute acid pretreated and enzymatically saccharified barley straw hydrolyzate (23.7g sugars/L) was 11.4g/L (0.48g/g available sugars, 0.26g/g straw) by the mixed sugar utilizing recombinant Escherichia coli strain FBR5 in 17 hours. The ethanol yields were 11.4 and 11.9g/L from 24.4 and 26.2g sugars/L obtained from lime and alkaline peroxide pretreated barley straw, respectively. No inhibition of fermentation occurred by any of the three pretreatments under the conditions used. |
| | |
Authors:
|
Badal C Saha; Michael A Cotta |
Related Documents
:
|
12860023 - Synthesis, characterization, and use of 2-[(2h(9))butoxy]acetic acid and 2-(3-methylbut... 18397253 - Response surface methodology to optimize the nutritional parameters for enhanced produc... 8478793 - Steroid metabolism with intestinal microorganisms. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article Date: 2009-10-27 |
Journal Detail:
|
Title: New biotechnology Volume: 27 ISSN: 1876-4347 ISO Abbreviation: N Biotechnol Publication Date: 2010 Feb |
Date Detail:
|
Created Date: 2010-03-01 Completed Date: 2010-05-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 10-6 Citation Subset: IM |
Copyright Information:
|
Published by Elsevier B.V. |
Affiliation:
|
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U. S. Department of Agriculture, Peoria, IL 61604, USA. Badal.Saha@ars.usda.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cellulase
/
metabolism* Cellulose / metabolism Escherichia coli / metabolism Ethanol / chemistry, metabolism* Fermentation* Glycoside Hydrolases / metabolism Hordeum* / anatomy & histology, chemistry, metabolism Hydrogen-Ion Concentration Industrial Microbiology / methods* Plant Stems* / chemistry, metabolism beta-Glucosidase / metabolism |
| Chemical | |
Reg. No./Substance:
|
64-17-5/Ethanol; 9004-34-6/Cellulose; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/hemicellulase; EC 3.2.1.21/beta-Glucosidase; EC 3.2.1.4/Cellulase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cyclodextrin-functionalized biomaterials loaded with miconazole prevent Candida albicans biofilm for...
Next Document: Comparative varicella vaccine effectiveness during outbreaks in day-care centres.