Document Detail


Comparison of pregnancy diagnosis in dairy cattle by use of a commercial ELISA and palpation per rectum.
MedLine Citation:
PMID:  19650701     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare agreement between 2 pregnancy tests in dairy cattle. DESIGN: Evaluation study. ANIMALS: 976 and 507 cattle for phases 1 and 2, respectively. PROCEDURES: Blood samples were collected, and palpation per rectum (PPR) was performed on cattle. Blood samples for the pregnancy-specific protein B (PSPB) ELISA were sent by courier to a commercial laboratory with results returned later. Results of PPR were extracted from herd records. Statistical comparison of results was performed by use of a mixed linear model and N analysis. RESULTS: Of 571 cattle classified as pregnant by the PSPB ELISA in phase 1, 30 (5%) were nonpregnant by PPR. Mean +/- SE adjusted optical density (OD) of cattle classified pregnant by both tests was significantly higher (0.31 +/- 0.01), compared with the adjusted OD of cattle classified pregnant by the PSPB ELISA and nonpregnant by PPR (0.22 +/- 0.02). Of 255 cows classified pregnant by the PSPB ELISA in phase 2, 31 (12%) were nonpregnant by PPR. Mean +/- SE adjusted OD of cattle classified pregnant by both tests was significantly higher (0.26 +/- 0.01), compared with the adjusted OD of cattle classified pregnant by the PSPB ELISA and nonpregnant by PPR (0.21 +/- 0.01). The N value was 0.82 and 0.81 for phases 1 and 2, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Good agreement existed between the 2 tests, especially at longer intervals after insemination. Discrepant results appeared to be attributable to a nonviable fetus, embryonic loss, or fetal loss.
Authors:
Matthew W Breed; Charles L Guard; Maurice E White; Mary C Smith; Lorin D Warnick
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Veterinary Medical Association     Volume:  235     ISSN:  0003-1488     ISO Abbreviation:  J. Am. Vet. Med. Assoc.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-04     Completed Date:  2009-09-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503067     Medline TA:  J Am Vet Med Assoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  292-8     Citation Subset:  IM    
Affiliation:
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / blood
Cattle / blood,  physiology*
Digital Rectal Examination / methods,  veterinary*
Enzyme-Linked Immunosorbent Assay / methods,  veterinary*
Evaluation Studies as Topic
Female
Fetal Viability / physiology
Pregnancy
Pregnancy Tests / methods,  standards,  veterinary*
Pregnancy-Specific beta 1-Glycoproteins / analysis*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Pregnancy-Specific beta 1-Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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