| Comparison of poultry exudate and carcass rinse sampling methods for the recovery of Campylobacter spp. subtypes demonstrates unique subtypes recovered from exudate. | |
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MedLine Citation:
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PMID: 18495278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The carcass rinse procedure is a method commonly used for the detection of Campylobacter spp. on processed poultry products. Alternatively, carcass exudate (weep or drip), a viscous fluid comprised of blood and water that leaks into packaging, can also be sampled. It is unknown however if direct carcass rinse or exudate/weep can be utilized to preferentially recover different Campylobacter spp. subtypes. If there is a difference in subtypes recovered, the Campylobacter spp. subtypes from carcass rinse analysis may not be indicative of consumer exposure, as the exudate is the fluid to which consumers are potentially exposed to due to kitchen cross-contamination. Experiments were conducted to determine if there are differences in recovery of Campylobacter spp. subtypes between the two methodologies. The experiment was performed in triplicate using three flocks located on different farms. For each flock, 50 fecal samples were obtained on the farm, 25 carcass rinses during pre-chill processing, 25 carcass rinses during post-chill processing, and 50 samples from exudate from carcasses stored at 4 degrees C (25 after 2-day storage and 25 after 6-day storage). Each sample type was cultured for Campylobacter spp. Isolates recovered from positive samples were subtyped using flaA SVR (flagellin A-short variable region) DNA sequence typing and compared for relatedness. The data demonstrated that multiple subtypes of Campylobacter jejuni were present in a flock, and that subtypes present in a flock during production were also present on the final processed product. Subtypes recovered by the two recovery methodologies were similar based on flaA SVR classification. Combining the totals from all 3 flocks a total of 10 flaA SVR subtypes were recovered from post-chill carcass rinses and 9 subtypes recovered from 6-day exudate samples. |
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Authors:
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M Simmons; K L Hiett; N J Stern; J F Frank |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-25 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 74 ISSN: 0167-7012 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-06-20 Completed Date: 2008-09-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 89-93 Citation Subset: IM |
Affiliation:
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University of Georgia, Department of Food Science and Technology, Athens, GA, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU526351; EU526352; EU526353; EU526354; EU526355; EU526356; EU526357; EU526358; EU526359; EU526360; EU526361; EU526362; EU526363; EU526364; EU526365; EU526366; EU526367; EU526368; EU526369; EU526370; EU526371; EU526372; EU526373; EU526374; EU526375; EU526376; EU526377; EU526378; EU526379; EU526380 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Typing Techniques Campylobacter / classification*, genetics, isolation & purification* Cluster Analysis DNA, Bacterial / chemistry, genetics Exudates and Transudates / microbiology* Feces / microbiology Flagellin / genetics Food Microbiology Molecular Sequence Data Poultry / microbiology Poultry Products / microbiology* Sequence Analysis, DNA Sequence Homology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 12777-81-0/Flagellin; 133606-66-3/flaA protein, bacteria |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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