| Comparison of a poly-L-lactide-co-ε-caprolactone and human amniotic membrane for urothelium tissue engineering applications. | |
| | |
MedLine Citation:
|
PMID: 21106575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The reconstructive surgery of urothelial defects, such as severe hypospadias is susceptible to complications. The major problem is the lack of suitable grafting materials. Therefore, finding alternative treatments such as reconstruction of urethra using tissue engineering is essential. The aim of this study was to compare the effects of naturally derived acellular human amniotic membrane (hAM) to synthetic poly-L-lactide-co-ε-caprolactone (PLCL) on human urothelial cell (hUC) viability, proliferation and urothelial differentiation level. The viability of cells was evaluated using live/dead staining and the proliferation was studied using WST-1 measurement. Cytokeratin (CK)7/8 and CK19 were used to confirm that the hUCs maintained their phenotype on different biomaterials. On the PLCL, the cell number significantly increased during the culturing period, in contrast to the hAM, where hUC proliferation was the weakest at 7 and 14 days. In addition, the majority of cells were viable and maintained their phenotype when cultured on PLCL and cell culture plastic, whereas on the hAM, the viability of hUCs decreased with time and the cells did not maintain their phenotype. The PLCL membranes supported the hUC proliferation significantly more than the hAM. These results revealed the significant potential of PLCL membranes in urothelial tissue engineering applications. |
| | |
Authors:
|
Reetta Sartoneva; Suvi Haimi; Susanna Miettinen; Bettina Mannerström; Anne-Marie Haaparanta; George K Sándor; Minna Kellomäki; Riitta Suuronen; Tuija Lahdes-Vasama |
Related Documents
:
|
15869425 - Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an... 16046665 - Adult bone marrow stem/progenitor cells (mscs) are preconditioned by microenvironmental... 18458385 - Comparison of two types of alginate microcapsules on stability and biocompatibility in ... 18202975 - Characterization of mesenchymal stromal cells derived from full-term umbilical cord blood. 8536935 - In vitro secretion of insulin-like growth factor-binding proteins from liver of striped... 10451225 - Effects of granulosa cells, cumulus cells, and oocyte density on in vitro fertilization... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-24 |
Journal Detail:
|
Title: Journal of the Royal Society, Interface / the Royal Society Volume: 8 ISSN: 1742-5662 ISO Abbreviation: J R Soc Interface Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-03-23 Completed Date: 2011-07-15 Revised Date: 2012-09-24 |
Medline Journal Info:
|
Nlm Unique ID: 101217269 Medline TA: J R Soc Interface Country: England |
Other Details:
|
Languages: eng Pagination: 671-7 Citation Subset: IM |
Affiliation:
|
Regea-Institute for Regenerative Medicine, University of Tampere, Tampere, Finland. reetta.sartoneva@regea.fi |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amnion
/
metabolism* Cell Survival Flow Cytometry / methods Humans Keratin-19 / metabolism Keratin-7 / metabolism Keratin-8 / metabolism Phenotype Polyesters / chemistry* Surface Properties Time Factors Tissue Engineering / methods* Urothelium / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Keratin-19; 0/Keratin-7; 0/Keratin-8; 0/Polyesters; 70524-20-8/lactide-caprolactone copolymer |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Substrate-induced phenotypic switches of human smooth muscle cells: an in vitro study of in-stent re...
Next Document: Effects of epidural analgesia on uterine artery Doppler in labour.