Document Detail

Comparison of phosphotungstate and dextran sulfate-Mg2+ precipitation procedures for determination of high density lipoprotein cholesterol.
MedLine Citation:
PMID:  6203665     Owner:  NLM     Status:  MEDLINE    
Because of the controversy over the best method for assaying high density lipoprotein (HDL) cholesterol in the clinical laboratory, a commonly used phosphotungstate method for precipitating low density and very low density lipoproteins (LDL and VLDL) was compared with a recently recommended dextran sulfate precipitation method. The accuracy and precision of HDL cholesterol determinations were similar for both methods. Either of these procedures would appear to be equally satisfactory for the assay of HDL cholesterol in the clinical laboratory.
G E Austin; E Maznicki; D Sgoutas
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical biochemistry     Volume:  17     ISSN:  0009-9120     ISO Abbreviation:  Clin. Biochem.     Publication Date:  1984 Jun 
Date Detail:
Created Date:  1984-07-27     Completed Date:  1984-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  166-9     Citation Subset:  IM    
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MeSH Terms
Apolipoprotein A-I
Apolipoproteins / blood
Apolipoproteins B
Cholesterol / blood*
Cholesterol, HDL
Cholesterol, LDL
Cholesterol, VLDL
Dextran Sulfate
Lipoproteins, HDL / blood*
Lipoproteins, LDL / blood
Lipoproteins, VLDL / blood
Phosphotungstic Acid*
Triglycerides / blood
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins; 0/Apolipoproteins B; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Cholesterol, VLDL; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 0/Triglycerides; 12067-99-1/Phosphotungstic Acid; 57-88-5/Cholesterol; 7439-95-4/Magnesium; 9004-54-0/Dextrans; 9042-14-2/Dextran Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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