Document Detail

Comparison of phosphodiesterase isozymes in rodent parotid glands.
MedLine Citation:
PMID:  10665368     Owner:  NLM     Status:  MEDLINE    
We investigated phosphodiesterase (PDE) isozymes, which hydrolyze cAMP, in rodent parotid glands (mouse, hamster and guinea pig) in order to clarify the effects of cGMP and Ca/calmodulin on the regulation of cellular cAMP and compared them with those of the rat. More than 80% of the activities were in the supernatant fractions except for the hamster. The isozymes were fractionated using Mono Q ion-exchange column. The mouse parotid PDEs consisted of PDE1 (Ca/calmodulin-dependent), PDE2 (cGMP-stimulated), PDE3 (cGMP-inhibited) and PDE4 (cAMP-specific) similar to those of the rat. PDE3 was not detected in the hamster, and PDE4 was not detected in the guinea pig. PDE activities in the supernatant of the mouse and the hamster were stimulated by cGMP, and that of the guinea pig was stimulated by Ca/calmodulin. These results suggest that various PDE isozymes are present in the parotid gland of several species of order Rodentia. There seems to be differences among the species with regard to the PDE isozymes.
A Imai; T Nashida; H Shimomura
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  124     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-03-23     Completed Date:  2000-03-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  397-403     Citation Subset:  IM    
Department of Oral Biochemistry, Nippon Dental University, School of Dentistry, Niigata, Japan.
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MeSH Terms
Calcium / metabolism,  pharmacology
Calmodulin / metabolism,  pharmacology
Chromatography, Ion Exchange
Cyclic AMP / metabolism
Cyclic AMP-Dependent Protein Kinases / metabolism
Cyclic GMP / metabolism,  pharmacology
Enzyme Activation
Guinea Pigs
Isoenzymes / drug effects,  genetics,  isolation & purification,  metabolism
Mice, Inbred Strains
Parotid Gland / drug effects,  enzymology*
Phosphoric Diester Hydrolases / drug effects,  genetics,  isolation & purification,  metabolism*
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction / methods
Species Specificity
Subcellular Fractions
Reg. No./Substance:
0/Calmodulin; 0/Isoenzymes; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; 7665-99-8/Cyclic GMP; EC AMP-Dependent Protein Kinases; EC 3.1.4.-/Phosphoric Diester Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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