Document Detail

Comparison of performance of Chirobiotic T, T2 and TAG columns in the separation of beta2- and beta3-homoamino acids.
MedLine Citation:
PMID:  18956385     Owner:  NLM     Status:  MEDLINE    
The enantiomers of eight unusual beta(2)- and beta(3)-homoamino acids were separated on chiral stationary phases containing the macrocyclic glycopeptide antibiotic teicoplanin (Chirobiotic T or T2) or teicoplanin aglycone (Chirobiotic TAG) as chiral selectors. The effects of the organic modifier, the mobile phase composition, and temperature on the separations were investigated. Linear van't Hoff plots were observed in the studied temperature range, 280-318 K, and the changes in enthalpy, Delta(DeltaH(o)), entropy, Delta(DeltaS(o)), and free energy, Delta(DeltaG(o)) were calculated. The values of the thermodynamic parameters depended on the nature of the selectors, the structures of the analytes, and especially the positions of the substituents on the analytes. A comparison of the separation performances of the macrocyclic glycopeptide stationary phases revealed that the Chirobiotic TAG column exhibited much better selectivity for beta(2)-homoamino acids, while the separation of beta(3)-homoamino acid enantiomers was better on Chirobiotic T or T2. The elution sequence was determined in some cases and was observed to be R < S.
Zoltán Pataj; István Ilisz; Robert Berkecz; Aleksandra Misicka; Dagmara Tymecka; Ferenc Fülöp; Daniel W Armstrong; Antal Péter
Related Documents :
16837005 - Separation and determination of perfluorinated carboxylic acids using capillary zone el...
17097095 - Enantiodifferentiation of n-benzyloxycarbonylaminophosphonic and phosphinic acids and t...
9376715 - Electrokinetic separation of enantiomers using a capillary coated with poly-terguride.
21275885 - Mild mitochondrial uncoupling as a therapeutic strategy.
2807145 - The "survival hormones": azelaic and pimelic acids, suppress the stress elicited by iso...
19378165 - Identification of nucleic acid high-affinity binding sequences of proteins by selex.
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  31     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-17     Completed Date:  2009-01-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  3688-97     Citation Subset:  IM    
Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / chemistry,  isolation & purification*
Chromatography, High Pressure Liquid / instrumentation*,  methods
Molecular Structure
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Buffers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Headspace single drop and hollow fiber liquid phase microextractions for HPLC determination of pheno...
Next Document:  Simultaneous enantioseparation of four beta2-agonists by capillary electrophoresis with cyclodextrin...